EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.75 | Ca2+ | - |
Streptomyces sp. | |
3.1.1.75 | DTT | - |
Streptomyces sp. | |
3.1.1.75 | K+ | - |
Streptomyces sp. | |
3.1.1.75 | Mg2+ | - |
Streptomyces sp. | |
3.1.1.75 | additional information | no inhibition of activity by azide | Streptomyces sp. | |
3.1.1.75 | Na+ | - |
Streptomyces sp. | |
3.1.1.75 | PMSF | - |
Streptomyces sp. | |
3.1.1.75 | Tween 80 | - |
Streptomyces sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.75 | 0.67 | - |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) | pH 8.0, 30°C | Streptomyces sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.75 | extracellular | - |
Streptomyces sp. | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.75 | 62000 | - |
x * 62000, about, SDS-PAGE and mass spectrometry | Streptomyces sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.75 | poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces sp. | - |
? | - |
? | |
3.1.1.75 | poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces sp. IN1 | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.75 | Streptomyces sp. | - |
soil isolate | - |
3.1.1.75 | Streptomyces sp. IN1 | - |
soil isolate | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.75 | native enzyme 401fold by ammonium sulfate fractionation and gel filtration | Streptomyces sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.75 | 12030 | - |
purified native enzyme, substrate poly(3-hydroxybutyrate-co-3-hydroxyvalerate), pH 8.0, 30°C | Streptomyces sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.75 | 4-nitrophenyl butyrate + H2O | - |
Streptomyces sp. | 4-nitrophenol + butyrate | - |
? | |
3.1.1.75 | 4-nitrophenyl butyrate + H2O | - |
Streptomyces sp. IN1 | 4-nitrophenol + butyrate | - |
? | |
3.1.1.75 | poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces sp. | ? | - |
? | |
3.1.1.75 | poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces sp. IN1 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.75 | ? | x * 62000, about, SDS-PAGE and mass spectrometry | Streptomyces sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.75 | P(3HB-co-3HV) depolymerase | - |
Streptomyces sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.75 | 80 | - |
- |
Streptomyces sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.75 | 80 | - |
purified enzyme, 15 min, stable | Streptomyces sp. |
3.1.1.75 | 90 | - |
almost complete deactivation of the purified enzyme after 15 min | Streptomyces sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.75 | 12 | - |
- |
Streptomyces sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.75 | 7 | 12 | the enzyme is completely inactivated at pH values below pH 7, but shows optimum activity at pH 12 | Streptomyces sp. |