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Literature summary extracted from

  • Miyazaki, T.; Matsumoto, Y.; Matsuda, K.; Kurakata, Y.; Matsuo, I.; Ito, Y.; Nishikawa, A.; Tonozuka, T.
    Heterologous expression and characterization of processing alpha-glucosidase I from Aspergillus brasiliensis ATCC 9642 (2011), Glycoconj. J., 28, 563-571.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.106 expressed in Escherichia coli strain JM109 Aspergillus brasiliensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.106 1-deoxynojirimycin
-
 Aspergillus brasiliensis
3.2.1.106 kojibiose
-
 Aspergillus brasiliensis
3.2.1.106 nigerose
-
 Aspergillus brasiliensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.106 0.0042
-
 Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine in 20 mM sodium phosphate, pH 7.0, at 25°C Aspergillus brasiliensis
3.2.1.106 0.0061
-
 Glc3Man9GlcNAc2-pyridylamine in 20 mM sodium phosphate, pH 7.0, at 25°C Aspergillus brasiliensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.106 Aspergillus brasiliensis G1UJA9 formerly Aspergillus niger
-
3.2.1.106 Aspergillus brasiliensis ATCC 9642 G1UJA9 formerly Aspergillus niger
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.106 glutathione-agarose column chromatography, gel filtration Aspergillus brasiliensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc2-pyridylamine + H2O
-
 Aspergillus brasiliensis Glc2Man9GlcNAc2-pyridylamine + D-glucose
-
 ?
3.2.1.106 Glc3Man9GlcNAc2-pyridylamine + H2O
-
 Aspergillus brasiliensis ATCC 9642 Glc2Man9GlcNAc2-pyridylamine + D-glucose
-
 ?
3.2.1.106 Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine + H2O
-
 Aspergillus brasiliensis Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine + D-glucose
-
 ?
3.2.1.106 Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine + H2O
-
 Aspergillus brasiliensis ATCC 9642 Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine + D-glucose
-
 ?
3.2.1.106 additional information the enzyme does not hydrolyze the terminal alpha(1-2)-linkage of Glc2Man9GlcNAc2-pyridylamine Aspergillus brasiliensis ?
-
 ?
3.2.1.106 additional information the enzyme does not hydrolyze the terminal alpha(1-2)-linkage of Glc2Man9GlcNAc2-pyridylamine Aspergillus brasiliensis ATCC 9642 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.106 PGI
-
 Aspergillus brasiliensis
3.2.1.106 processing alpha-glucosidase I
-
 Aspergillus brasiliensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.106 1100
-
 Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine in 20 mM sodium phosphate, pH 7.0, at 25°C Aspergillus brasiliensis
3.2.1.106 1450
-
 Glc3Man9GlcNAc2-pyridylamine in 20 mM sodium phosphate, pH 7.0, at 25°C Aspergillus brasiliensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.106 7
-
-
 Aspergillus brasiliensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.106 6 8 the enzyme retains 89% and 94% of maximum activity at pH 6.0 and 8.0, respectively Aspergillus brasiliensis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.106 0.02
-
 in 20 mM sodium phosphate, pH 7.0, at 25°C Aspergillus brasiliensis 1-deoxynojirimycin
3.2.1.106 0.02
-
 in 20 mM sodium phosphate, pH 7.0, at 25°C Aspergillus brasiliensis kojibiose
3.2.1.106 1.9
-
 in 20 mM sodium phosphate, pH 7.0, at 25°C Aspergillus brasiliensis nigerose