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Literature summary extracted from

  • Zhang, L.; Thiewes, H.; van Kan, J.A.
    The D-galacturonic acid catabolic pathway in Botrytis cinerea (2011), Fungal Genet. Biol., 48, 990-997.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.365 expressed in Escherichia coli BL21 cells Botrytis cinerea
4.2.1.146 gene Bclgd1, quantitative RT-PCR expression analysis Botrytis cinerea

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.146 additional information gene replacement strategy for generating Bortrytis cinerea deletion constructs, complementation study, overview. Targeted gene replacement of all four genes, either separately or in combinations, yields mutants that are affected in growth on D-galacturonic acid, pectate, or pectin as the sole carbon source Botrytis cinerea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.365 0.12
-
 D-glucuronate isoform GAR1, 10 mM sodium phosphate, pH 7.0, at 22°C Botrytis cinerea
1.1.1.365 1
-
 D-glucuronate isoform GAR2, 10 mM sodium phosphate, pH 7.0, at 22°C Botrytis cinerea
1.1.1.365 1.4
-
 D-galacturonate isoform GAR2, 10 mM sodium phosphate, pH 7.0, at 22°C Botrytis cinerea
1.1.1.365 2.5
-
 D-galacturonate isoform GAR1, 10 mM sodium phosphate, pH 7.0, at 22°C Botrytis cinerea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.365 36900
-
 x * 36900, isoform GAR1, calculated from amino acid sequence Botrytis cinerea
1.1.1.365 49200
-
 x * 49200, isoform GAR2, calculated from amino acid sequence Botrytis cinerea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.365 D-galacturonate + NADPH + H+ Botrytis cinerea
-
 L-galactonate + NADP+
-
 r
1.1.1.365 D-galacturonate + NADPH + H+ Botrytis cinerea B05.10
-
 L-galactonate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.365 Botrytis cinerea
-
-
-
1.1.1.365 Botrytis cinerea B05.10
-
-
-
4.2.1.6 no activity in Botrytis cinerea
-
-
-
4.2.1.146 Botrytis cinerea
-
 gene Bclgd1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.365 D-galacturonate + NADPH + H+
-
 Botrytis cinerea L-galactonate + NADP+
-
 r
1.1.1.365 D-galacturonate + NADPH + H+
-
 Botrytis cinerea B05.10 L-galactonate + NADP+
-
 r
1.1.1.365 D-glucuronate + NADPH + H+
-
 Botrytis cinerea ?
-
 r
1.1.1.365 D-glucuronate + NADPH + H+
-
 Botrytis cinerea B05.10 ?
-
 r
4.2.1.146 L-galactonate
-
 Botrytis cinerea 2-keto-3-deoxy-L-galactonate + H2O
-
 ?
4.2.1.146 L-galactonate
-
 Botrytis cinerea B05.10 2-keto-3-deoxy-L-galactonate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.365 ? x * 36900, isoform GAR1, calculated from amino acid sequence Botrytis cinerea
1.1.1.365 ? x * 49200, isoform GAR2, calculated from amino acid sequence Botrytis cinerea

Synonyms

EC Number Synonyms Comment Organism
1.1.1.365 gar1 isoform Botrytis cinerea
1.1.1.365 GAR2 isoform Botrytis cinerea
4.2.1.146 LGD1
-
 Botrytis cinerea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.146 22
-
 assay at room temperature Botrytis cinerea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.146 5.2
-
 assay at Botrytis cinerea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.365 NADPH
-
 Botrytis cinerea

Expression

EC Number Organism Comment Expression
1.1.1.365 Botrytis cinerea isoform GAR1 is induced about 10fold, while isoform GAR2 is strongly induced in cultures with D-galacturonic acid, pectate, and pectin as carbon source at 3 h after transfer, compared to continuous growth in glucose-containing culture up
4.2.1.146 Botrytis cinerea pectate, pectinate, and galacturonic acid suppress enmzyme expression down
4.2.1.146 Botrytis cinerea glucose induces the enzyme up

General Information

EC Number General Information Comment Organism
4.2.1.146 metabolism the enzyme catalyzes the second step in the D-galacturonic acid catabolic pathway of the fungus Botrytis cinerea
4.2.1.146 additional information the virulence of all Bortrytis cinerea mutants in the D-galacturonic acid catabolic pathway on tomato leaves, apple fruit and bell peppers is unaltered Botrytis cinerea