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Literature summary extracted from

  • Hartl, L.; Gastebois, A.; Aimanianda, V.; Latge, J.P.
    Characterization of the GPI-anchored endo beta-1,3-glucanase Eng2 of Aspergillus fumigatus (2011), Fungal Genet. Biol., 48, 185-191.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 expression in Pichia pastoris Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 additional information
-
 additional information KM value for carboxymethyl curdlan-Remazol Brilliant Blue 0.8 mg/ml, pH 6.2, 37°C Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 65993
-
 x * 65993, calculated, x * 150000, SDS-PAGE of recombinant protein, due to hyperglycosylation Aspergillus fumigatus
3.2.1.39 150000
-
 x * 65993, calculated, x * 150000, SDS-PAGE of recombinant protein, due to hyperglycosylation Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Aspergillus fumigatus
-
 member of glycosyl hydrolase family 16
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.39 glycoprotein sequence analysis reveals two potential N-glycosylation sites at positions N200GSS and N453GTS Aspergillus fumigatus
3.2.1.39 phosphoprotein sequence analysis reveals a GPI modification site at position LSN630GA Aspergillus fumigatus
3.2.1.39 proteolytic modification sequence analysis reveals a signal peptide cleavage site at position VSA21-T22A Aspergillus fumigatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 fruit body of Neosartorya fumigata Aspergillus fumigatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 0.18
-
 substrate curdlan, pH 6.2, 37°C Aspergillus fumigatus
3.2.1.39 0.23
-
 substrate lichenan, pH 6.2, 37°C Aspergillus fumigatus
3.2.1.39 0.71
-
 substrate laminarin, pH 6.2, 37°C Aspergillus fumigatus
3.2.1.39 0.86
-
 substrate laminarioligosaccharide, pH 6.2, 37°C Aspergillus fumigatus
3.2.1.39 1.16
-
 substrate laminarihexaose, pH 6.2, 37°C Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 carboxymethyl curdlan-Remazol Brilliant Blue + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.39 curdlan + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.39 laminarin + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.39 laminaritetraose + H2O smallest substrate accepted Aspergillus fumigatus ?
-
 ?
3.2.1.39 lichenan + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.39 additional information enzyme preferentially acts on soluble beta-1,3-glucans. Additional transferase activity for oligosaccharides of a low degree of polymerization Aspergillus fumigatus ?
-
 ?
3.2.1.39 reduced laminarihexaose + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.39 reduced laminarioligosaccharide + H2O 19 glucose units Aspergillus fumigatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 65993, calculated, x * 150000, SDS-PAGE of recombinant protein, due to hyperglycosylation Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 Eng2
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 24 40 wide Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 5 6.5 wide Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
3.2.1.39 physiological function growth of a deletion strain in various media in solid and liquid cultures at different temperatures does not show a growth phenotype Aspergillus fumigatus