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Literature summary extracted from

  • Imaram, W.; Saylor, B.T.; Centonze, C.P.; Richards, N.G.; Angerhofer, A.
    EPR spin trapping of an oxalate-derived free radical in the oxalate decarboxylase reaction (2011), Free Radic. Biol. Med., 50, 1009-1015.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.2 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.2 S161A the mutant shows increased specific activity compared to the wild type enzyme Bacillus subtilis
4.1.1.2 S161T the mutant shows reduced specific activity compared to the wild type enzyme Bacillus subtilis
4.1.1.2 T165S the mutant shows reduced specific activity compared to the wild type enzyme Bacillus subtilis
4.1.1.2 T165V the mutant shows strongly reduced specific activity compared to the wild type enzyme Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.2 Mn2+ the wild type enzyme contains 1.4 Mn2+ ions per monomer Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.2 Bacillus subtilis O34714
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.2 Ni-NTA column chromatography, gel filtration Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.2 4.5
-
 mutant enzyme T165V, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication Bacillus subtilis
4.1.1.2 22
-
 mutant enzyme T165S, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication Bacillus subtilis
4.1.1.2 29
-
 mutant enzyme S161T, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication Bacillus subtilis
4.1.1.2 40
-
 wild type enzyme, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication Bacillus subtilis
4.1.1.2 48
-
 mutant enzyme S161A, in 50 mM sodium acetate, pH 4.2, temperature not specified in the publication Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.2 oxalate + H+
-
 Bacillus subtilis formate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.2 OXDC
-
 Bacillus subtilis