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Literature summary extracted from

  • Kataoka, M.; Wada, M.; Nishi, K.; Yamada, H.; Shimizu, S.
    Purification and characterization of L-allo-threonine aldolase from Aeromonas jandaei DK-39 (1997), FEMS Microbiol. Lett., 151, 245-248.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.49 4-chloromercuribenzoate 1 mM, 76% inhibition Aeromonas jandaei
4.1.2.49 5,5'-dithiobis(2-nitrobenzoic acid) 1 mM, 100% inhibition Aeromonas jandaei
4.1.2.49 Acriflavine 1 mM, 65% inhibition Aeromonas jandaei
4.1.2.49 Ag2SO4 1 mM, 100% inhibition Aeromonas jandaei
4.1.2.49 AlCl3 1 mM, 69% inhibition Aeromonas jandaei
4.1.2.49 CdCl2 1 mM, 80% inhibition Aeromonas jandaei
4.1.2.49 D-(-)-penicillamine 1 mM, 87% inhibition Aeromonas jandaei
4.1.2.49 FeCl3 1 mM, 100% inhibition Aeromonas jandaei
4.1.2.49 FeSO4 1 mM, 100% inhibition Aeromonas jandaei
4.1.2.49 HgCl2 1 mM, 99% inhibition Aeromonas jandaei
4.1.2.49 hydroxylamine 1 mM, 100% inhibition Aeromonas jandaei
4.1.2.49 iodoacetamide 1 mM, 56% inhibition Aeromonas jandaei
4.1.2.49 N-bromosuccinimide 1 mM, 89% inhibition Aeromonas jandaei
4.1.2.49 phenylhydrazine 1 mM, 91% inhibition Aeromonas jandaei
4.1.2.49 Semicarbazide 1 mM, 63% inhibition Aeromonas jandaei
4.1.2.49 ZnSO4 1 mM, 98% inhibition Aeromonas jandaei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.49 1.45
-
 L-allo-threonine pH 7.4, temperature not specified in the publication Aeromonas jandaei
4.1.2.49 6.77
-
 DL-threo-3,4-Dihydroxyphenylserine pH 7.4, temperature not specified in the publication Aeromonas jandaei
4.1.2.49 19.6
-
 DL-threo-beta-phenylserine pH 7.4, temperature not specified in the publication Aeromonas jandaei
4.1.2.49 27.8
-
 DL-beta-hydroxynorvaline pH 7.4, temperature not specified in the publication Aeromonas jandaei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.49 38000
-
 4 * 38000, SDS-PAGE Aeromonas jandaei
4.1.2.49 160000
-
 gel filtration Aeromonas jandaei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.49 L-allo-threonine Aeromonas jandaei
-
 glycine + acetaldehyde
-
 ?
4.1.2.49 L-allo-threonine Aeromonas jandaei DK-39
-
 glycine + acetaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.49 Aeromonas jandaei O07051
-
-
4.1.2.49 Aeromonas jandaei DK-39 O07051
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.49
-
 Aeromonas jandaei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.49 37.1
-
 DL-beta-hydroxynorvaline, pH 7.4, temperature not specified in the publication Aeromonas jandaei
4.1.2.49 45.2
-
 L-allo-threonine, pH 7.4, temperature not specified in the publication Aeromonas jandaei
4.1.2.49 208
-
 DL-threo-beta-phenylserine, pH 7.4, temperature not specified in the publication Aeromonas jandaei
4.1.2.49 217
-
 DL-threo-3,4-dihydroxyphenylserine, pH 7.4, temperature not specified in the publication Aeromonas jandaei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.49 DL-beta-hydroxynorvaline + H2O Vmax/KM is 4% compared to DL-threo-3,4-dihydroxyphenylserine Aeromonas jandaei glycine + propionaldehyde
-
 ?
4.1.2.49 DL-beta-hydroxynorvaline + H2O Vmax/KM is 4% compared to DL-threo-3,4-dihydroxyphenylserine Aeromonas jandaei DK-39 glycine + propionaldehyde
-
 ?
4.1.2.49 DL-threo-3,4-dihydroxyphenylserine + H2O
-
 Aeromonas jandaei glycine + 3,4-dihydroxybenzaldehyde
-
 ?
4.1.2.49 DL-threo-3,4-dihydroxyphenylserine + H2O
-
 Aeromonas jandaei DK-39 glycine + 3,4-dihydroxybenzaldehyde
-
 ?
4.1.2.49 DL-threo-beta-phenylserine + H2O Vmax/KM is 33% compared to DL-threo-3,4-dihydroxyphenylserine Aeromonas jandaei glycine + benzaldehyde
-
 ?
4.1.2.49 L-allo-threonine
-
 Aeromonas jandaei glycine + acetaldehyde
-
 ?
4.1.2.49 L-allo-threonine Vmax/KM is 97% compared to DL-threo-3,4-dihydroxyphenylserine Aeromonas jandaei glycine + acetaldehyde
-
 ?
4.1.2.49 L-allo-threonine
-
 Aeromonas jandaei DK-39 glycine + acetaldehyde
-
 ?
4.1.2.49 L-allo-threonine Vmax/KM is 97% compared to DL-threo-3,4-dihydroxyphenylserine Aeromonas jandaei DK-39 glycine + acetaldehyde
-
 ?
4.1.2.49 additional information no activity with L-threonine or L-serine Aeromonas jandaei ?
-
 ?
4.1.2.49 additional information no activity with L-threonine or L-serine Aeromonas jandaei DK-39 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.49 tetramer 4 * 38000, SDS-PAGE Aeromonas jandaei

Synonyms

EC Number Synonyms Comment Organism
4.1.2.49 L-allo-threonine acetaldehyde-lyase
-
 Aeromonas jandaei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.49 7.4
-
 assay at Aeromonas jandaei

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.49 pyridoxal 5'-phosphate required Aeromonas jandaei