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Literature summary extracted from

  • Joesaar, M.; Heinaru, E.; Viggor, S.; Vedler, E.; Heinaru, A.
    Diversity of the transcriptional regulation of the pch gene cluster in two indigenous p-cresol-degradative strains of Pseudomonas fluorescens (2010), FEMS Microbiol. Ecol., 72, 464-475.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.17.9.1 analysis the enzyme is used as a tool to characterize catabolic differences between phenol- and p-cresol-degrading Pseudomonas fluorescens strains PC18 and PC24, overview Pseudomonas fluorescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.9.1 the pchACXF operon encodes p-hydroxybenzaldehyde dehydrogenase and PCMH, mapping of the transcriptional start sites of the pch operons, DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic analysis, gene pchF expression in Escherichia coli strain DH5alpha Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.9.1 4-methylphenol + acceptor + H2O Pseudomonas fluorescens
-
 4-hydroxybenzaldehyde + reduced acceptor
-
 ?
1.17.9.1 4-methylphenol + acceptor + H2O Pseudomonas fluorescens PC24
-
 4-hydroxybenzaldehyde + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.9.1 Pseudomonas fluorescens C6JUF6 gene pchF
-
1.17.9.1 Pseudomonas fluorescens C6JUG1 gene pchF
-
1.17.9.1 Pseudomonas fluorescens PC24 C6JUF6 gene pchF
-
1.17.9.1 Pseudomonas fluorescens PC24 C6JUG1 gene pchF
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.9.1 4-methylphenol + acceptor + H2O
-
 Pseudomonas fluorescens 4-hydroxybenzaldehyde + reduced acceptor
-
 ?
1.17.9.1 4-methylphenol + acceptor + H2O
-
 Pseudomonas fluorescens PC24 4-hydroxybenzaldehyde + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.9.1 p-cresolmethylhydroxylase
-
 Pseudomonas fluorescens
1.17.9.1 PCMH
-
 Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.9.1 7.5
-
 assay at Pseudomonas fluorescens

Expression

EC Number Organism Comment Expression
1.17.9.1 Pseudomonas fluorescens different induction patterns of strains PC18 and PC24 are caused by differences in the PchR regulators of these strains additional information
1.17.9.1 Pseudomonas fluorescens PCMH of strain PC18 is induced during growth on phenol up

General Information

EC Number General Information Comment Organism
1.17.9.1 metabolism PCMH is a key enzyme responsible for the catabolism of p-cresol via the protocatechuate ortho pathway, pathway overview Pseudomonas fluorescens
1.17.9.1 additional information transcriptional regulation of the pchACXF operon by PchR, a putative sigma54-dependent regulator, overview. Phenol does not activate the regulator of PC24, and both regulators respond similarly to p-cresol Pseudomonas fluorescens
1.17.9.1 additional information transcriptional regulation of the pchACXF operon by PchR, a putative sigma54-dependent regulator, overview. Phenol is a more efficient effector for PchR of PC18 than p-cresol Pseudomonas fluorescens