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Literature summary extracted from

  • Lee, S.J.; Kim, H.S.; Kim, d.o..J.; Yoon, H.J.; Kim, K.H.; Yoon, J.Y.; Suh, S.W.
    Crystal structures of LacD from Staphylococcus aureus and LacD.1 from Streptococcus pyogenes: insights into substrate specificity and virulence gene regulation (2011), FEBS Lett., 585, 307-312.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.40 expressed in Escherichia coli Rosetta2(DE3) cells Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.40 sitting drop vapor diffusion method Staphylococcus aureus
4.1.2.40 sitting drop vapor diffusion method, using Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.40 L165E the mutant shows enhanced substrate specificity toward fructose-1,6-bisphosphate Staphylococcus aureus
4.1.2.40 L165E/L275S the mutant shows enhanced substrate specificity toward fructose-1,6-bisphosphate Staphylococcus aureus
4.1.2.40 L275S the mutant shows enhanced substrate specificity toward fructose-1,6-bisphosphate Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.40 Staphylococcus aureus Q5HE13
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.40 nickel affinity column chromatography, HiTrap-Q column chromatography, and Superdex 200 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.40 D-tagatose 1,6-bisphosphate
-
 Staphylococcus aureus glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.40 dimer x-ray crystallography Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
4.1.2.40 LacD a class I tagatose-1,6-bisphosphate aldolase Staphylococcus aureus
4.1.2.40 tagatose-1,6-bisphosphate aldolase
-
 Staphylococcus aureus
4.1.2.40 TBP aldolase
-
 Staphylococcus aureus