BRENDA - Enzyme Database show

Molecular characterization of an oxidosqualene cyclase that yields shionone, a unique tetracyclic triterpene ketone of Aster tataricus

Sawai, S.; Uchiyama, H.; Mizuno, S.; Aoki, T.; Akashi, T.; Ayabe, S.; Takahashi, T.; FEBS Lett. 585, 1031-1036 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.99.46
expressed in lanosterol synthase-deficient yeast; expressed in Saccharomyces cerevisiae strain GIL77 (lanosterol synthase-deficient strain)
Aster tataricus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.99.39
(S)-2,3-oxidosqualene
Aster tataricus
-
beta-amyrin
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.99.39
Aster tataricus
-
-
-
5.4.99.46
Aster tataricus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.4.99.39
flower
-
Aster tataricus
-
5.4.99.39
leaf
; of root and leaf of stem
Aster tataricus
-
5.4.99.39
radical leaf
-
Aster tataricus
-
5.4.99.39
rhizome
-
Aster tataricus
-
5.4.99.39
root
-
Aster tataricus
-
5.4.99.39
stem
-
Aster tataricus
-
5.4.99.39
stolon
-
Aster tataricus
-
5.4.99.46
flower
; high expression
Aster tataricus
-
5.4.99.46
leaf
; high expression
Aster tataricus
-
5.4.99.46
radical leaf
-
Aster tataricus
-
5.4.99.46
rhizome
-
Aster tataricus
-
5.4.99.46
root
-
Aster tataricus
-
5.4.99.46
stem
; high expression
Aster tataricus
-
5.4.99.46
stolon
-
Aster tataricus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.39
(S)-2,3-oxidosqualene
-
715030
Aster tataricus
beta-amyrin
-
-
-
?
5.4.99.46
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
715030
Aster tataricus
shionone + beta-amyrin + friedelin + dammara-20,24-dienol + 4-epishionone
the ratio of the products is roughly estimated as shionone:beta-amyrin:friedelin:dammara-20,24-dienol:4-epishionone = 90%:2%:2%:1.5%:1.5%
-
-
?
5.4.99.46
oxidosqualene
-
715030
Aster tataricus
shionone
the cyclase yields ca. 90% shionone and small amounts of beta-amyrin, friedelin, dammara-20,24-dienol, and 4-epishionone
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.46
expressed in lanosterol synthase-deficient yeast; expressed in Saccharomyces cerevisiae strain GIL77 (lanosterol synthase-deficient strain)
Aster tataricus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.99.39
(S)-2,3-oxidosqualene
Aster tataricus
-
beta-amyrin
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.4.99.39
flower
-
Aster tataricus
-
5.4.99.39
leaf
; of root and leaf of stem
Aster tataricus
-
5.4.99.39
radical leaf
-
Aster tataricus
-
5.4.99.39
rhizome
-
Aster tataricus
-
5.4.99.39
root
-
Aster tataricus
-
5.4.99.39
stem
-
Aster tataricus
-
5.4.99.39
stolon
-
Aster tataricus
-
5.4.99.46
flower
; high expression
Aster tataricus
-
5.4.99.46
leaf
; high expression
Aster tataricus
-
5.4.99.46
radical leaf
-
Aster tataricus
-
5.4.99.46
rhizome
-
Aster tataricus
-
5.4.99.46
root
-
Aster tataricus
-
5.4.99.46
stem
; high expression
Aster tataricus
-
5.4.99.46
stolon
-
Aster tataricus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.39
(S)-2,3-oxidosqualene
-
715030
Aster tataricus
beta-amyrin
-
-
-
?
5.4.99.46
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
715030
Aster tataricus
shionone + beta-amyrin + friedelin + dammara-20,24-dienol + 4-epishionone
the ratio of the products is roughly estimated as shionone:beta-amyrin:friedelin:dammara-20,24-dienol:4-epishionone = 90%:2%:2%:1.5%:1.5%
-
-
?
5.4.99.46
oxidosqualene
-
715030
Aster tataricus
shionone
the cyclase yields ca. 90% shionone and small amounts of beta-amyrin, friedelin, dammara-20,24-dienol, and 4-epishionone
-
-
?