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Literature summary extracted from

  • Shibuya, M.; Nishimura, K.; Yasuyama, N.; Ebizuka, Y.
    Identification and characterization of glycosyltransferases involved in the biosynthesis of soyasaponin I in Glycine max (2010), FEBS Lett., 584, 2258-2264.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.272 expression in Escherichia coli Glycine max
2.4.1.273 expression in Escherichia coli Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.272 0.012
-
 soyasapogenol B 3-O-beta-D-glucuronide pH 7.4, 37°C Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.272 55866
-
 x * 55866, calculated from sequence Glycine max
2.4.1.273 53518
-
 x * 53518, calculated from sequence Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.272 UDP-galactose + soyasapogenol B 3-O-beta-D-glucuronide Glycine max the enzyme is involved in the biosynthesis of soyasaponin I in Glycine max UDP + soyasaponin III
-
 ?
2.4.1.273 UDP-rhamnose + soyasaponin III Glycine max the enzyme is involved in the biosynthesis of soyasaponin I in Glycine max UDP + soyasaponin I
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.272 Glycine max D4Q9Z4
-
-
2.4.1.273 Glycine max D4Q9Z5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.272 UDP-galactose + soyasapogenol B 3-O-beta-D-glucuronide the enzyme is involved in the biosynthesis of soyasaponin I in Glycine max Glycine max UDP + soyasaponin III
-
 ?
2.4.1.272 UDP-galactose + soyasapogenol B 3-O-beta-D-glucuronide high substrate specificity for soyasapogenol B 3-O-beta-D-glucuronide Glycine max UDP + soyasaponin III soyasaponin III i.e. 3beta-(2-O-beta-D-galactopyranosyl-beta-D-glucopyranosyloxyuronic acid)olean-12-ene-22beta,24-diol ?
2.4.1.273 UDP-rhamnose + soyasaponin III the enzyme is involved in the biosynthesis of soyasaponin I in Glycine max Glycine max UDP + soyasaponin I
-
 ?
2.4.1.273 UDP-rhamnose + soyasaponin III soyasaponin III i.e. 3beta-(2-O-beta-D-galactopyranosyl-beta-D-glucopyranosyloxyuronic acid)olean-12-ene-22beta,24-diol. The enzyme has strong sugar donor specificity for UDP-rhamnose. No activity with UDP-glucose, UDP-galactose or UDPglucuronic acid Glycine max UDP + soyasaponin I
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.272 ? x * 55866, calculated from sequence Glycine max
2.4.1.273 ? x * 53518, calculated from sequence Glycine max

Synonyms

EC Number Synonyms Comment Organism
2.4.1.272 GmSGT2
-
 Glycine max
2.4.1.272 UDP-galactose:SBMG-galactosyltransferase
-
 Glycine max
2.4.1.272 UGT73P2
-
 Glycine max
2.4.1.273 GmSGT3
-
 Glycine max
2.4.1.273 UGT91H4
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.272 37
-
 assay at Glycine max
2.4.1.273 37
-
 assay at Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.272 7.4
-
 assay at Glycine max
2.4.1.273 7.4
-
 assay at Glycine max

General Information

EC Number General Information Comment Organism
2.4.1.272 physiological function part of the biosynthetic pathway for soyasaponins Glycine max
2.4.1.273 physiological function part of the biosynthetic pathway for soyasaponins Glycine max