Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dolzan, M.; Johansson, K.; Roig-Zamboni, V.; Campanacci, V.; Tegoni, M.; Schneider, G.; Cambillau, C.
    Crystal structure and reactivity of YbdL from Escherichia coli identify a methionine aminotransferase function (2004), FEBS Lett., 571, 141-146.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.88 expressed in Escherichia coli C41 (DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.88 hanging drop vapor diffusion method, using 0.2 M NaCl, 0.1 M sodium/potassium phosphate, pH 6.3, and 23.5% (w/v) PEG1000, at 20°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.88 45647
-
 2 * 45647, X-ray crystallography Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.88 Escherichia coli P77806
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.88 Ni2+ affinity column chromatography and Superdex 200 pg gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.88 L-histidine + a 2-oxo acid 88% activity compared to L-methionine (at 1 mM) Escherichia coli ?
-
 ?
2.6.1.88 L-leucine + a 2-oxo acid 11% activity compared to L-methionine (at 1 mM) Escherichia coli ?
-
 ?
2.6.1.88 L-methionine + a 2-oxo acid the enzyme shows a preference for L-methionine (100% activity at 1 mM L-methionine) Escherichia coli ?
-
 ?
2.6.1.88 L-phenylalanine + a 2-oxo acid 57% activity compared to L-methionine (at 1 mM) Escherichia coli ?
-
 ?
2.6.1.88 L-tyrosine + a 2-oxo acid 9% activity compared to L-methionine (at 1 mM) Escherichia coli ?
-
 ?
2.6.1.88 additional information the enzyme does not use L-tryptophan, L-valine, L-asparagine, L-glutamate, and L-arginine as substrates Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.88 homodimer 2 * 45647, X-ray crystallography Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.6.1.88 methionine aminotransferase
-
 Escherichia coli
2.6.1.88 YbdL
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.88 pyridoxal 5'-phosphate dependent on. The active site contains a bound pyridoxal 5'-phosphate, covalently attached to the conserved active site lysine residue Lys236 Escherichia coli