EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.58 | 0.003 | - |
benzoyl-CoA | - |
Pseudomonas sp. | |
1.14.13.58 | 0.125 | - |
NADPH | - |
Pseudomonas sp. | |
6.2.1.25 | 0.009 | - |
benzoate | anaerobically induced enzyme | Pseudomonas sp. | |
6.2.1.25 | 0.011 | - |
benzoate | benzoate, , aerobically induced enzyme | Pseudomonas sp. | |
6.2.1.25 | 0.089 | - |
CoA | anaerobically induced enzyme | Pseudomonas sp. | |
6.2.1.25 | 0.09 | - |
2-Fluorobenzoate | aerobically induced enzyme | Pseudomonas sp. | |
6.2.1.25 | 0.1 | - |
CoASH | 2-fluorobenzoate, , anaerobically induced enzyme | Pseudomonas sp. | |
6.2.1.25 | 0.105 | - |
CoA | aerobically induced enzyme | Pseudomonas sp. | |
6.2.1.25 | 0.125 | - |
ATP | anaerobically induced enzyme | Pseudomonas sp. | |
6.2.1.25 | 0.277 | - |
ATP | aerobically induced enzyme | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.2.1.25 | 54000 | - |
2 * 54000, SDS-PAGE, anaerobically induced enzyme | Pseudomonas sp. |
6.2.1.25 | 56000 | - |
2 * 56000, SDS-PAGE, aerobically induced enzyme | Pseudomonas sp. |
6.2.1.25 | 120000 | - |
gel filtration, anaerobically induced enzyme | Pseudomonas sp. |
6.2.1.25 | 130000 | - |
gel filtration, aerobically induced enzyme | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.58 | Benzoyl-CoA + NADPH + O2 | Pseudomonas sp. | enzyme is specifically induced during aerobic growth with benzoate | ? | - |
? | |
1.14.13.58 | Benzoyl-CoA + NADPH + O2 | Pseudomonas sp. | second enzyme in a variant of the aerobic benzoate degradation pathway | ? | - |
? | |
1.14.13.58 | Benzoyl-CoA + NADPH + O2 | Pseudomonas sp. KB740 | enzyme is specifically induced during aerobic growth with benzoate | ? | - |
? | |
1.14.13.58 | Benzoyl-CoA + NADPH + O2 | Pseudomonas sp. KB740 | second enzyme in a variant of the aerobic benzoate degradation pathway | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.58 | Pseudomonas sp. | - |
- |
- |
1.14.13.58 | Pseudomonas sp. KB740 | - |
- |
- |
6.2.1.25 | Pseudomonas sp. | - |
- |
- |
6.2.1.25 | Pseudomonas sp. | - |
an anaerobically induced benzoate-CoA ligase and an aerobically induced benzoate-CoA ligase with different properties | - |
6.2.1.25 | Pseudomonas sp. KB740 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.58 | partial | Pseudomonas sp. |
6.2.1.25 | - |
Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.58 | 0.025 | - |
- |
Pseudomonas sp. |
6.2.1.25 | 73 | - |
- |
Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.58 | Benzoyl-CoA + NADPH + O2 | enzyme is specifically induced during aerobic growth with benzoate | Pseudomonas sp. | ? | - |
? | |
1.14.13.58 | Benzoyl-CoA + NADPH + O2 | second enzyme in a variant of the aerobic benzoate degradation pathway | Pseudomonas sp. | ? | - |
? | |
1.14.13.58 | Benzoyl-CoA + NADPH + O2 | enzyme is specifically induced during aerobic growth with benzoate | Pseudomonas sp. KB740 | ? | - |
? | |
1.14.13.58 | Benzoyl-CoA + NADPH + O2 | second enzyme in a variant of the aerobic benzoate degradation pathway | Pseudomonas sp. KB740 | ? | - |
? | |
6.2.1.25 | ATP + 2-aminobenzoate + CoA | - |
Pseudomonas sp. | AMP + diphosphate + 2-aminobenzoyl-CoA | - |
? | |
6.2.1.25 | ATP + 2-fluorobenzoate + CoA | - |
Pseudomonas sp. | AMP + diphosphate + 2-fluorobenzoyl-CoA | - |
? | |
6.2.1.25 | ATP + 2-fluorobenzoate + CoA | - |
Pseudomonas sp. KB740 | AMP + diphosphate + 2-fluorobenzoyl-CoA | - |
? | |
6.2.1.25 | ATP + 2-methylbenzoate + CoA | no activity with anaerobically induced enzyme, 15% of the activity relative to benzoate with the aerobically induced enzyme | Pseudomonas sp. | AMP + diphosphate + 2-methylbenzoyl-CoA | - |
? | |
6.2.1.25 | ATP + 3-fluorobenzoate + CoA | - |
Pseudomonas sp. | AMP + diphosphate + 3-fluorobenzoyl-CoA | - |
? | |
6.2.1.25 | ATP + 4-fluorobenzoate + CoA | - |
Pseudomonas sp. | AMP + diphosphate + 4-fluorobenzoyl-CoA | - |
? | |
6.2.1.25 | ATP + benzoate + CoA | - |
Pseudomonas sp. | AMP + diphosphate + benzoyl-CoA | - |
? | |
6.2.1.25 | ATP + benzoate + CoA | - |
Pseudomonas sp. KB740 | AMP + diphosphate + benzoyl-CoA | - |
? | |
6.2.1.25 | additional information | not 3-hydroxybenzoate | Pseudomonas sp. | ? | - |
? | |
6.2.1.25 | additional information | not 3-hydroxybenzoate | Pseudomonas sp. KB740 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.2.1.25 | dimer | 2 * 54000, SDS-PAGE, anaerobically induced enzyme | Pseudomonas sp. |
6.2.1.25 | dimer | 2 * 56000, SDS-PAGE, aerobically induced enzyme | Pseudomonas sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.25 | additional information | - |
additional information | - |
Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.25 | 9 | - |
aerobically induced enzyme | Pseudomonas sp. |
6.2.1.25 | 9.3 | - |
anaerobically induced enzyme | Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.58 | FAD | required | Pseudomonas sp. | |
1.14.13.58 | FAD | Km: 0.15 mM | Pseudomonas sp. | |
1.14.13.58 | NADPH | required | Pseudomonas sp. |