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Literature summary extracted from

  • Altenschmidt, U.; Oswald, B.; Steiner, E.; Herrmann, H.; Fuchs, G.
    New aerobic benzoate oxidation pathway via benzoyl-coenzyme A and 3-hydroxybenzoyl-coenzyme A in a denitrifying Pseudomonas sp. (1993), J. Bacteriol., 175, 4851-4858.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.58 0.003
-
 benzoyl-CoA
-
 Pseudomonas sp.
1.14.13.58 0.125
-
 NADPH
-
 Pseudomonas sp.
6.2.1.25 0.009
-
 benzoate anaerobically induced enzyme Pseudomonas sp.
6.2.1.25 0.011
-
 benzoate benzoate, , aerobically induced enzyme Pseudomonas sp.
6.2.1.25 0.089
-
 CoA anaerobically induced enzyme Pseudomonas sp.
6.2.1.25 0.09
-
 2-Fluorobenzoate aerobically induced enzyme Pseudomonas sp.
6.2.1.25 0.1
-
 CoASH 2-fluorobenzoate, , anaerobically induced enzyme Pseudomonas sp.
6.2.1.25 0.105
-
 CoA aerobically induced enzyme Pseudomonas sp.
6.2.1.25 0.125
-
 ATP anaerobically induced enzyme Pseudomonas sp.
6.2.1.25 0.277
-
 ATP aerobically induced enzyme Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.25 54000
-
 2 * 54000, SDS-PAGE, anaerobically induced enzyme Pseudomonas sp.
6.2.1.25 56000
-
 2 * 56000, SDS-PAGE, aerobically induced enzyme Pseudomonas sp.
6.2.1.25 120000
-
 gel filtration, anaerobically induced enzyme Pseudomonas sp.
6.2.1.25 130000
-
 gel filtration, aerobically induced enzyme Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.58 Benzoyl-CoA + NADPH + O2 Pseudomonas sp. enzyme is specifically induced during aerobic growth with benzoate ?
-
 ?
1.14.13.58 Benzoyl-CoA + NADPH + O2 Pseudomonas sp. second enzyme in a variant of the aerobic benzoate degradation pathway ?
-
 ?
1.14.13.58 Benzoyl-CoA + NADPH + O2 Pseudomonas sp. KB740 enzyme is specifically induced during aerobic growth with benzoate ?
-
 ?
1.14.13.58 Benzoyl-CoA + NADPH + O2 Pseudomonas sp. KB740 second enzyme in a variant of the aerobic benzoate degradation pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.58 Pseudomonas sp.
-
-
-
1.14.13.58 Pseudomonas sp. KB740
-
-
-
6.2.1.25 Pseudomonas sp.
-
-
-
6.2.1.25 Pseudomonas sp.
-
 an anaerobically induced benzoate-CoA ligase and an aerobically induced benzoate-CoA ligase with different properties
-
6.2.1.25 Pseudomonas sp. KB740
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.58 partial Pseudomonas sp.
6.2.1.25
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.58 0.025
-
-
 Pseudomonas sp.
6.2.1.25 73
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.58 Benzoyl-CoA + NADPH + O2 enzyme is specifically induced during aerobic growth with benzoate Pseudomonas sp. ?
-
 ?
1.14.13.58 Benzoyl-CoA + NADPH + O2 second enzyme in a variant of the aerobic benzoate degradation pathway Pseudomonas sp. ?
-
 ?
1.14.13.58 Benzoyl-CoA + NADPH + O2 enzyme is specifically induced during aerobic growth with benzoate Pseudomonas sp. KB740 ?
-
 ?
1.14.13.58 Benzoyl-CoA + NADPH + O2 second enzyme in a variant of the aerobic benzoate degradation pathway Pseudomonas sp. KB740 ?
-
 ?
6.2.1.25 ATP + 2-aminobenzoate + CoA
-
 Pseudomonas sp. AMP + diphosphate + 2-aminobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 2-fluorobenzoate + CoA
-
 Pseudomonas sp. AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 2-fluorobenzoate + CoA
-
 Pseudomonas sp. KB740 AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 2-methylbenzoate + CoA no activity with anaerobically induced enzyme, 15% of the activity relative to benzoate with the aerobically induced enzyme Pseudomonas sp. AMP + diphosphate + 2-methylbenzoyl-CoA
-
 ?
6.2.1.25 ATP + 3-fluorobenzoate + CoA
-
 Pseudomonas sp. AMP + diphosphate + 3-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 4-fluorobenzoate + CoA
-
 Pseudomonas sp. AMP + diphosphate + 4-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + benzoate + CoA
-
 Pseudomonas sp. AMP + diphosphate + benzoyl-CoA
-
 ?
6.2.1.25 ATP + benzoate + CoA
-
 Pseudomonas sp. KB740 AMP + diphosphate + benzoyl-CoA
-
 ?
6.2.1.25 additional information not 3-hydroxybenzoate Pseudomonas sp. ?
-
 ?
6.2.1.25 additional information not 3-hydroxybenzoate Pseudomonas sp. KB740 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.25 dimer 2 * 54000, SDS-PAGE, anaerobically induced enzyme Pseudomonas sp.
6.2.1.25 dimer 2 * 56000, SDS-PAGE, aerobically induced enzyme Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.25 additional information
-
 additional information
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.25 9
-
 aerobically induced enzyme Pseudomonas sp.
6.2.1.25 9.3
-
 anaerobically induced enzyme Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.58 FAD required Pseudomonas sp.
1.14.13.58 FAD Km: 0.15 mM Pseudomonas sp.
1.14.13.58 NADPH required Pseudomonas sp.