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Literature summary extracted from

  • Stern, A.L.; Naworyta, A.; Cazzulo, J.J.; Mowbray, S.L.
    Structures of type B ribose 5-phosphate isomerase from Trypanosoma cruzi shed light on the determinants of sugar specificity in the structural family (2011), FEBS J., 278, 793-808.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.6 expressed in Escherichia coli ER2566 cells Trypanosoma cruzi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.6 RpiB (wild type or C69A mutant enzyme), sitting drop vapor diffusion method, using 0.5% (v/v) Jeffamine ED-2001, 0.1 M HEPES, 1.1 M Na-malonate (pH 7.0) for the wild type enzyme in complex with phosphate, or 0.8 M Na?K phosphate (pH 8.2) for the mutant enzyme C69A in complex with phosphate, or 20% (w/v) poly(ethyleneglycol) 550 monomethyl ether, 0.1 M NaCl, 0.1 M bicine (pH 9.0) for the wild type enzyme in complex with D-ribose 5-phosphate, or 20% (w/v) poly(ethylene glycol) 6000, 0.2 M ammonium chloride, 0.1 M Tris-HCl (pH 8.0) for wild type enzyme in complex with 4-phospho-D-erythronohydroxamic acid, or 20% (w/v) poly(ethylene glycol) 3350, 0.2 M sodium acetate, 0.1 M bis-Tris propane (pH 8.5) for mutant enzyme C69A in complex with allose 6-phosphate Trypanosoma cruzi

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.6 C69A inactive Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.6 D-Allose 6-phosphate weak competitive inhibitor Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.6 5
-
D-ribulose 5-phosphate in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.6 Trypanosoma cruzi Q4CQE2
-
-
5.3.1.6 Trypanosoma cruzi CL Brener Q4CQE2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.6 Superdex 75 gel filtration Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.6 D-ribulose 5-phosphate
-
Trypanosoma cruzi D-ribose 5-phosphate
-
?
5.3.1.6 D-ribulose 5-phosphate
-
Trypanosoma cruzi CL Brener D-ribose 5-phosphate
-
?
5.3.1.6 additional information the enzyme cannot isomerizes D-allose 6-phosphate Trypanosoma cruzi ?
-
?
5.3.1.6 additional information the enzyme cannot isomerizes D-allose 6-phosphate Trypanosoma cruzi CL Brener ?
-
?

Subunits

EC Number Subunits Comment Organism
5.3.1.6 tetramer x-ray crystallography Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
5.3.1.6 ribose-5-phosphate isomerase
-
Trypanosoma cruzi
5.3.1.6 RpiB
-
Trypanosoma cruzi
5.3.1.6 type B ribose 5-phosphate isomerase
-
Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.6 2 8 D-ribulose 5-phosphate in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C Trypanosoma cruzi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.1.6 15
-
D-Allose 6-phosphate in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C Trypanosoma cruzi