BRENDA - Enzyme Database

The gene carD encodes the aldehyde dehydrogenase responsible for neurosporaxanthin biosynthesis in Fusarium fujikuroi

Diaz-Sanchez, V.; Estrada, A.F.; Trautmann, D.; Al-Babili, S.; Avalos, J.; FEBS J. 278, 3164-3176 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.2.1.82
carD expressed in Escherichia coli BL21 strain
Fusarium fujikuroi
1.2.1.82
generation of an Escherichia coli strain producing beta-apo-4'-carotenal, which is converted into neurosporaxanthin upon expression of carD
Fusarium fujikuroi
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.2.1.82
beta-apo-4'-carotenal + NAD+ + H2O
Fusarium fujikuroi
carD function substantiated by its targeted disruption in a Fusarium fujikuroi carotenoid-overproducing strain, which resulted in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal
beta-apo-4'-carotenoic acid + NADH + H+
beta-apo-4'-carotenoic acid i.e. neurosporaxanthin
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.2.1.82
Fusarium fujikuroi
F6IBC7
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.2.1.82
partial purification by generating of a crude lysate for in vitro assays
Fusarium fujikuroi
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.2.1.82
apo-8'-lycopenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
apo-8'-lycopenoic acid + NADH + 2 H+
-
-
-
?
1.2.1.82
apo-8'-lycopenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
apo-8'-lycopenoic acid + NADH + H+
-
-
-
?
1.2.1.82
beta-apo-10'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-10'-carotenoic acid + NADH + H+
-
-
-
?
1.2.1.82
beta-apo-10'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-10'-carotenoic acid + NADH + 2 H+
-
-
-
?
1.2.1.82
beta-apo-4'-carotenal + NAD+ + H2O
carD function substantiated by its targeted disruption in a Fusarium fujikuroi carotenoid-overproducing strain, which resulted in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal
714988
Fusarium fujikuroi
beta-apo-4'-carotenoic acid + NADH + H+
beta-apo-4'-carotenoic acid i.e. neurosporaxanthin
-
-
?
1.2.1.82
beta-apo-4'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
neurosporaxanthin + NADH + 2 H+
-
-
-
?
1.2.1.82
beta-apo-8'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-8'-carotenoic acid + NADH + H+
-
-
-
?
1.2.1.82
beta-apo-8'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-8'-carotenoic acid + NADH + 2 H+
-
-
-
?
1.2.1.82
additional information
no substrate: retinal
714988
Fusarium fujikuroi
?
-
-
-
?
1.2.1.82
additional information
C20-retinal i.e. beta-apo-15'-carotenal is not converted by carD enzymatic activity, indicating the requirement for a minimal length of the substrate chain
714988
Fusarium fujikuroi
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.2.1.82
CarD
-
Fusarium fujikuroi
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.82
carD expressed in Escherichia coli BL21 strain
Fusarium fujikuroi
1.2.1.82
generation of an Escherichia coli strain producing beta-apo-4'-carotenal, which is converted into neurosporaxanthin upon expression of carD
Fusarium fujikuroi
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.2.1.82
beta-apo-4'-carotenal + NAD+ + H2O
Fusarium fujikuroi
carD function substantiated by its targeted disruption in a Fusarium fujikuroi carotenoid-overproducing strain, which resulted in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal
beta-apo-4'-carotenoic acid + NADH + H+
beta-apo-4'-carotenoic acid i.e. neurosporaxanthin
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.82
partial purification by generating of a crude lysate for in vitro assays
Fusarium fujikuroi
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.2.1.82
apo-8'-lycopenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
apo-8'-lycopenoic acid + NADH + 2 H+
-
-
-
?
1.2.1.82
apo-8'-lycopenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
apo-8'-lycopenoic acid + NADH + H+
-
-
-
?
1.2.1.82
beta-apo-10'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-10'-carotenoic acid + NADH + H+
-
-
-
?
1.2.1.82
beta-apo-10'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-10'-carotenoic acid + NADH + 2 H+
-
-
-
?
1.2.1.82
beta-apo-4'-carotenal + NAD+ + H2O
carD function substantiated by its targeted disruption in a Fusarium fujikuroi carotenoid-overproducing strain, which resulted in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal
714988
Fusarium fujikuroi
beta-apo-4'-carotenoic acid + NADH + H+
beta-apo-4'-carotenoic acid i.e. neurosporaxanthin
-
-
?
1.2.1.82
beta-apo-4'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
neurosporaxanthin + NADH + 2 H+
-
-
-
?
1.2.1.82
beta-apo-8'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-8'-carotenoic acid + NADH + H+
-
-
-
?
1.2.1.82
beta-apo-8'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-8'-carotenoic acid + NADH + 2 H+
-
-
-
?
1.2.1.82
additional information
no substrate: retinal
714988
Fusarium fujikuroi
?
-
-
-
?
1.2.1.82
additional information
C20-retinal i.e. beta-apo-15'-carotenal is not converted by carD enzymatic activity, indicating the requirement for a minimal length of the substrate chain
714988
Fusarium fujikuroi
?
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
1.2.1.82
Fusarium fujikuroi
CarD mRNA content is enhanced by light, and CarD mRNA levels are higher in carotenoid-overproducing mutants
up
1.2.1.82
Fusarium fujikuroi
carD mRNA levels increase about 3fold after 30 min of illumination
up
General Information
EC Number
General Information
Commentary
Organism
1.2.1.82
physiological function
targeted disruption of CarD in a Gibberella fujikuroi carotenoid-overproducing strain results in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal, its derivative beta-apo-4'-carotenol, and minor amounts of other carotenoids. Strains harboring the CarD mutation acquire a yellowish pigmentation
Fusarium fujikuroi
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.2.1.82
physiological function
targeted disruption of CarD in a Gibberella fujikuroi carotenoid-overproducing strain results in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal, its derivative beta-apo-4'-carotenol, and minor amounts of other carotenoids. Strains harboring the CarD mutation acquire a yellowish pigmentation
Fusarium fujikuroi
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.2.1.82
Fusarium fujikuroi
CarD mRNA content is enhanced by light, and CarD mRNA levels are higher in carotenoid-overproducing mutants
up
1.2.1.82
Fusarium fujikuroi
carD mRNA levels increase about 3fold after 30 min of illumination
up