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Literature summary extracted from

  • Liang, W.; Ouyang, S.; Shaw, N.; Joachimiak, A.; Zhang, R.; Liu, Z.J.
    Conversion of D-ribulose 5-phosphate to D-xylulose 5-phosphate: new insights from structural and biochemical studies on human RPE (2011), FASEB J., 25, 497-504.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.1 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.3.1 hanging drop vapor diffusion method, using 25% (w/v) PEG 3350, 100 mM Bis-Tris (pH 5.5), and 200 mM NaCl Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.1 D175A inactive Homo sapiens
5.1.3.1 D37A the mutation almost abolishes the enzymatic activity Homo sapiens
5.1.3.1 H35A the mutation almost abolishes the enzymatic activity Homo sapiens
5.1.3.1 H70A completely insoluble Homo sapiens
5.1.3.1 L12A the mutation results in more than 50% decrease in the activity Homo sapiens
5.1.3.1 M141A the mutation results in about 30% decrease in the activity Homo sapiens
5.1.3.1 M39A the mutation results in about 10% decrease in the activity Homo sapiens
5.1.3.1 M72A the mutation results in an almost 50% decrease in the activity Homo sapiens
5.1.3.1 S10A the mutation almost abolishes the enzymatic activity Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.1 Fe2+ RPE uses Fe2+ for catalysis Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.1 Homo sapiens Q96AT9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.1 Ni-affinity column chromatography and Superdex G75 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.3.1 brain
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.1 D-Ribulose 5-phosphate
-
Homo sapiens D-Xylulose 5-phosphate
-
r

Synonyms

EC Number Synonyms Comment Organism
5.1.3.1 D-ribulose 5-phosphate 3-epimerase
-
Homo sapiens
5.1.3.1 RPE
-
Homo sapiens