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Literature summary extracted from

  • Le, Y.; Peng, J.; Pei, J.; Li, H.; Duan, Z.; Shao, W.
    Properties of an NAD+-dependent DNA ligase from the hyperthermophile Thermotoga maritima and its application in PCR amplification of long DNA fragments (2010), Enzyme Microb. Technol., 46, 113-117.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.2 expressed in Escherichia coli BL21(DE3) cells Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.2 Ca2+ the enzyme requires a divalent cation like Ca2+, optimal activity at about 20 mM Ca2+ Thermotoga maritima
6.5.1.2 Mg2+ the enzyme requires a divalent cation like Mg2+, optimal activity at about 20 mM Mg2+ Thermotoga maritima
6.5.1.2 Mn2+ the enzyme requires a divalent cation like Mn2+, maximal activity at 3 mM Mn2+ Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.2 78000
-
 x * 78000, SDS-PAGE Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Thermotoga maritima
-
-
-
6.5.1.2 Thermotoga maritima ATCC 43589
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.2 heat treatment and immobilized metal affinity column chromatography Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 additional information purified DNA ligase exhibits activity on DNA fragments with cohesive termini, and no activity is detected on blunt-end DNA. DNA ligase promotes the amplification of long DNA fragments from the genomic DNA of Thermotoga maritima Thermotoga maritima ?
-
 ?
6.5.1.2 additional information purified DNA ligase exhibits activity on DNA fragments with cohesive termini, and no activity is detected on blunt-end DNA. DNA ligase promotes the amplification of long DNA fragments from the genomic DNA of Thermotoga maritima Thermotoga maritima ATCC 43589 ?
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Hind III digested plasmid Thermotoga maritima AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Hind III digested plasmid Thermotoga maritima ATCC 43589 AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.2 ? x * 78000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
6.5.1.2 LigA
-
 Thermotoga maritima
6.5.1.2 NAD+-dependent DNA ligase
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.2 60
-
 with Hind III digested plasmid as substrate Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.5.1.2 50 70
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.5.1.2 95
-
 the purified enzyme has a half-life of over 30 min at 95°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.2 8
-
 with Hind III digested plasmid as substrate Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.5.1.2 7 9
-
 Thermotoga maritima

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.5.1.2 7.2 8.8 the enzyme retains over 80% of its activity after holding a pH ranging from 7.2 to 8.8 for 1 h at 80°C Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.2 additional information does not require ATP as cofactor Thermotoga maritima
6.5.1.2 NAD+ required, optimal activity at 1 mM NAD+ Thermotoga maritima