Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Eady, N.A.; Jesmin, N.A.; Servos, S.; Cass, A.E.; Nagy, J.M.; Brown, K.A.
    Probing the function of Mycobacterium tuberculosis catalase-peroxidase by site-directed mutagenesis (2005), Dalton Trans., 2005, 3495-3500.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.11.1.21 medicine the enzyme activates the pro-antitubercular drug isoniazid Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.21 H108L the mutant shows decreased catalase catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 W107F the mutant shows decreased catalase catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 W107F/W321F the mutant shows decreased catalase catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 W321F the mutant shows decreased catalase catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.21 0.11
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 0.32
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 0.36
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 0.47
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 1.6
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 5.7
-
 tert-butyl hydroperoxide peroxidatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 12.7
-
 tert-butyl hydroperoxide peroxidatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 15.5
-
 tert-butyl hydroperoxide peroxidatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 31.5
-
 H2O2 catalatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 32.1
-
 H2O2 catalatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 33
-
 H2O2 catalatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 42.6
-
 H2O2 catalatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 54.9
-
 H2O2 catalatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 91.2
-
 tert-butyl hydroperoxide peroxidatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 97.9
-
 tert-butyl hydroperoxide peroxidatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + tert-butyl hydroperoxide
-
 Mycobacterium tuberculosis oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + ?
-
 ?
1.11.1.21 H2O2
-
 Mycobacterium tuberculosis O2 + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Mycobacterium tuberculosis oxidized o-dianisidine + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.21 0.34
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 0.37
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 0.72
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 2.4
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 4.8
-
 H2O2 catalatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 5.4
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 7.7
-
 H2O2 catalatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 12
-
 H2O2 catalatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 2300
-
 H2O2 catalatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 6800
-
 H2O2 catalatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme heme-dependent enzyme Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.21 0.014
-
 tert-butyl hydroperoxide peroxidatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 0.028
-
 tert-butyl hydroperoxide peroxidatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 0.029
-
 tert-butyl hydroperoxide peroxidatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 0.15
-
 H2O2 catalatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 0.21
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 0.22
-
 H2O2 catalatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 0.23
-
 H2O2 catalatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 0.42
-
 tert-butyl hydroperoxide peroxidatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 0.48
-
 tert-butyl hydroperoxide peroxidatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 1
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 5.1
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 6.3
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 17
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 73
-
 H2O2 catalatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.11.1.21 160
-
 H2O2 catalatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis