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Literature summary extracted from

  • Nihira, T.; Nakai, H.; Kitaoka, M.
    3-O-alpha-D-glucopyranosyl-L-rhamnose posphorylase from Clostridium phytofermentans (2012), Carbohydr. Res., 350, 94-97.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.282 expression in Escherichia coli Lachnoclostridium phytofermentans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.282 2.7
-
 L-rhamnose pH 6.5, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.282 9.6
-
 beta-D-glucose 1-phosphate pH 6.5, 30°C, reverse phosphorolysis reaction Lachnoclostridium phytofermentans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.282 cytosol
-
 Lachnoclostridium phytofermentans 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.282 87990
-
 calculated from amino acid sequence Lachnoclostridium phytofermentans
2.4.1.282 88000
-
 SDS-PAGE Lachnoclostridium phytofermentans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.282 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate Lachnoclostridium phytofermentans phosphorolysis reaction D-glucose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.282 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate Lachnoclostridium phytofermentans ATCC 700394 phosphorolysis reaction D-glucose + alpha-D-mannose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.282 Lachnoclostridium phytofermentans A9KM56
-
-
2.4.1.282 Lachnoclostridium phytofermentans ATCC 700394 A9KM56
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.282 3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate
-
 Lachnoclostridium phytofermentans L-rhamnose + beta-D-glucose 1-phosphate
-
 r
2.4.1.282 3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate
-
 Lachnoclostridium phytofermentans ATCC 700394 L-rhamnose + beta-D-glucose 1-phosphate
-
 r
2.4.1.282 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate phosphorolysis reaction Lachnoclostridium phytofermentans D-glucose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.282 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate phosphorolysis reaction Lachnoclostridium phytofermentans ATCC 700394 D-glucose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.282 L-rhamnose + beta-D-glucose 1-phosphate
-
 Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate
-
 r
2.4.1.282 L-rhamnose + beta-D-glucose 1-phosphate
-
 Lachnoclostridium phytofermentans ATCC 700394 3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate
-
 r
2.4.1.282 additional information The enzyme does not phosphorylyze alpha,alpha-trehalose, kojibiose, nigerose, maltose. In the reverse phosphorolysis reaction the enzyme is specific for L-rhamnose as acceptor and beta-D-glucose 1-phosphate as donor. Lachnoclostridium phytofermentans ?
-
 ?
2.4.1.282 additional information The enzyme does not phosphorylyze alpha,alpha-trehalose, kojibiose, nigerose, maltose. In the reverse phosphorolysis reaction the enzyme is specific for L-rhamnose as acceptor and beta-D-glucose 1-phosphate as donor. Lachnoclostridium phytofermentans ATCC 700394 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.282 cphy1019
-
 Lachnoclostridium phytofermentans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.282 30
-
-
 Lachnoclostridium phytofermentans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.282 30
-
 stable up to Lachnoclostridium phytofermentans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.282 1.6
-
 L-rhamnose pH 6.5, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.282 2.5
-
 beta-D-glucose 1-phosphate pH 6.5, 30°C, reverse phosphorolysis reaction Lachnoclostridium phytofermentans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.282 6.5
-
-
 Lachnoclostridium phytofermentans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.282 6 9
-
 Lachnoclostridium phytofermentans