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Literature summary extracted from

  • Li, L.; Jiang, X.; Guan, H.; Wang, P.
    Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216 (2011), Carbohydr. Res., 346, 794-800.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.3 extracellular
-
Pseudomonas sp.
-
-
4.2.2.11 extracellular
-
Pseudomonas sp.
-
-

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Pseudomonas sp.
-
-
-
4.2.2.3 Pseudomonas sp. HZJ 216
-
-
-
4.2.2.11 Pseudomonas sp.
-
-
-
4.2.2.11 Pseudomonas sp. HZJ 216
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 purification leads to a mixture of three monomeric alginate lyases with molecular masses of 60.25, 36, and 23 kDa Pseudomonas sp.
4.2.2.11 purification leads to a mixture of three monomeric alginate lyases with molecular masses of 60.25, 36, and 23 kDa Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alpha-L-mannuronyl linkage in alginate
-
Pseudomonas sp. ? products are six different di-and trisaccharides. The enzymatic hydrolysis occurs between two random guluronic acid or/and mannuronic acid residues, and produces one G residue or M residue on the reducing end and an unsaturated residue on the non-reducing end for all products ?
4.2.2.3 alpha-L-mannuronyl linkage in alginate
-
Pseudomonas sp. HZJ 216 ? products are six different di-and trisaccharides. The enzymatic hydrolysis occurs between two random guluronic acid or/and mannuronic acid residues, and produces one G residue or M residue on the reducing end and an unsaturated residue on the non-reducing end for all products ?
4.2.2.11 alpha-L-guluronosyl linkage in alginate
-
Pseudomonas sp. ? products are six different di-and trisaccharides. The enzymatic hydrolysis occurs between two random guluronic acid or/and mannuronic acid residues, and produces one G residue or M residue on the reducing end and an unsaturated residue on the non-reducing end for all products ?
4.2.2.11 alpha-L-guluronosyl linkage in alginate
-
Pseudomonas sp. HZJ 216 ? products are six different di-and trisaccharides. The enzymatic hydrolysis occurs between two random guluronic acid or/and mannuronic acid residues, and produces one G residue or M residue on the reducing end and an unsaturated residue on the non-reducing end for all products ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 More purification leads to a mixture of three monomeric alginate lyases with molecular masses of 60.25, 36, and 23 kDa Pseudomonas sp.
4.2.2.11 More purification leads to a mixture of three monomeric alginate lyases with molecular masses of 60.25, 36, and 23 kDa Pseudomonas sp.