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Literature summary extracted from

  • Yoshida, T.; Inami, Y.; Matsui, T.; Nagasawa, T.
    Regioselective carboxylation of catechol by 3,4-dihydroxybenzoate decarboxylase of Enterobacter cloacae P (2010), Biotechnol. Lett., 32, 701-705.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.1.63 addition of 5 mM dithiothreitol and 20 mM Na2S2O3 to a cell-free extract stabilizes the enzyme Enterobacter cloacae

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.63 Enterobacter cloacae
-
-
-
4.1.1.63 Enterobacter cloacae P241
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.63
-
 Enterobacter cloacae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
4.1.1.63 4°C, cell-free extract in the absence of sulfhydryl-protecting reagents, 2 h, 50% loss of activity Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.63 3,4-Dihydroxybenzoate 28% conversion after 14 h at 30°C Enterobacter cloacae Catechol + CO2
-
 ?
4.1.1.63 3,4-Dihydroxybenzoate 28% conversion after 14 h at 30°C Enterobacter cloacae P241 Catechol + CO2
-
 ?
4.1.1.63 catechol + KHCO3 28% conversion after 14 h at 30°C Enterobacter cloacae 3,4-dihydroxybenzoate
-
 ?
4.1.1.63 catechol + KHCO3 28% conversion after 14 h at 30°C Enterobacter cloacae P241 3,4-dihydroxybenzoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.63 3,4-dihydroxybenzoate decarboxylase
-
 Enterobacter cloacae

Expression

EC Number Organism Comment Expression
4.1.1.63 Enterobacter cloacae 3,4-dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 is induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium up