Literature summary extracted from
Kim, B.G.; Shin, K.H.; Lee, Y.; Hur, H.G.; Lim, Y.; Ahn, J.H.
Multiple regiospecific methylations of a flavonoid by plant O-methyltransferases expressed in E. coli (2005), Biotechnol. Lett., 27, 1861-1864.
Application
EC Number |
Application |
Comment |
Organism |
---|
2.1.1.231 |
synthesis |
coexpression of Oryza sativa ROMT-9, which methylates specifically at the 3'-hydroxyl group of quercetin and SOMT-2, which methylates at the 4'-hydroxyl group, in Escherichia coli. Reaction products of quercetin with the transformant show two methylation products that correspond to the 3'-methylated and the 3',4'-dimethylated quercetin. More than 90% of quercetin is converted into the 3',4'-dimethylated quercetin after 24 h incubation |
Glycine max |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.1.1.231 |
coexpression of Oryza sativa ROMT-9, which methylates specifically at the 3'-hydroxyl group of quercetin and SOMT-2, which methylates at the 4'-hydroxyl group, in Escherichia coli |
Glycine max |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.1.1.231 |
additional information |
coexpression of Oryza sativa ROMT-9, which methylates specifically at the 3'-hydroxyl group of quercetin and SOMT-2, which methylates at the 4'-hydroxyl group, in Escherichia coli. Reaction products of quercetin with the transformant show two methylation products that correspond to the 3'-methylated and the 3',4'-dimethylated quercetin. More than 90% of quercetin is converted into the 3',4'-dimethylated quercetin after 24 h incubation |
Glycine max |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.1.1.231 |
40000 |
- |
x * 40000, SDS-PAGE |
Glycine max |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.1.1.231 |
Glycine max |
- |
- |
- |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.1.1.231 |
? |
x * 40000, SDS-PAGE |
Glycine max |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.1.1.231 |
SOMT-2 |
- |
Glycine max |