EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.78 | synthesis | development of a fed-batch strategy for engineered enzyme, using high cell-density fermentation. Mannanase activity reaches 5069 U/ml after cultivation for 56 h in 50 l fermenter | Aspergillus niger |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.78 | expression in Pichia pastoris | Aspergillus niger |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.78 | additional information | gene optimization according to the codon usage bias in Pichia pastoris and synthesis by splicing overlap extension PCR | Aspergillus niger |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.78 | 37700 | - |
x * 37700, calculated | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.78 | Aspergillus niger | - |
- |
- |
3.2.1.78 | Aspergillus niger CBS 513.88 | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.78 | 1139 | - |
pH 5.0, 40°C | Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.78 | locust bean gum + H2O | - |
Aspergillus niger | ? | - |
? | |
3.2.1.78 | locust bean gum + H2O | - |
Aspergillus niger CBS 513.88 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.78 | ? | x * 37700, calculated | Aspergillus niger |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.78 | Man26A | - |
Aspergillus niger |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.78 | 45 | - |
- |
Aspergillus niger |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.78 | 5 | 60 | recombinant enzyme, more than 30% of maximum activity within | Aspergillus niger |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.78 | 90 | - |
half-life 15 min | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.78 | 5 | - |
- |
Aspergillus niger |