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Literature summary extracted from

  • Zhao, W.; Zheng, J.; Zhou, H.B.
    A thermotolerant and cold-active mannan endo-1,4-beta-mannosidase from Aspergillus niger CBS 513.88: Constitutive overexpression and high-density fermentation in Pichia pastoris (2011), Biores. Technol., 102, 7538-7547.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.78 synthesis development of a fed-batch strategy for engineered enzyme, using high cell-density fermentation. Mannanase activity reaches 5069 U/ml after cultivation for 56 h in 50 l fermenter Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Pichia pastoris Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.78 additional information gene optimization according to the codon usage bias in Pichia pastoris and synthesis by splicing overlap extension PCR Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 37700
-
x * 37700, calculated Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Aspergillus niger
-
-
-
3.2.1.78 Aspergillus niger CBS 513.88
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 1139
-
pH 5.0, 40°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 locust bean gum + H2O
-
Aspergillus niger ?
-
?
3.2.1.78 locust bean gum + H2O
-
Aspergillus niger CBS 513.88 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 37700, calculated Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 Man26A
-
Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 45
-
-
Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.78 5 60 recombinant enzyme, more than 30% of maximum activity within Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 90
-
half-life 15 min Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 5
-
-
Aspergillus niger