Any feedback?
Literature summary extracted from
- Characterization of the aldol condensation activity of the trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (tHBP-HA) cloned from Pseudomonas fluorescens N3 (2011), Biochim. Biophys. Acta, 1814, 622-629.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.2.45 | expressed in Escherichia coli JM109 (pVLT33-ALD) and M15 (pREP4) cells | Pseudomonas fluorescens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.45 | 3.58 | - |
Salicylaldehyde | at pH 7.0, 30°C | Pseudomonas fluorescens |  |
| 4.1.2.45 | 8.98 | - |
benzaldehyde | at pH 7.0, 30°C | Pseudomonas fluorescens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.45 | 38000 | - |
x * 38000, His-tagged recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.45 | Pseudomonas fluorescens | - |
- |
- |
| 4.1.2.45 | Pseudomonas fluorescens N3 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.2.45 | Co2+ Talon metal affinity resin column chromatography | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.45 | benzaldehyde + pyruvate | - |
Pseudomonas fluorescens | trans-benzylidenepyruvate | - |
? | |
| 4.1.2.45 | benzaldehyde + pyruvate | - |
Pseudomonas fluorescens N3 | trans-benzylidenepyruvate | - |
? | |
| 4.1.2.45 | salicylaldehyde + pyruvate | - |
Pseudomonas fluorescens | trans-o-hydroxybenzylidenepyruvate | - |
? | |
| 4.1.2.45 | salicylaldehyde + pyruvate | - |
Pseudomonas fluorescens N3 | trans-o-hydroxybenzylidenepyruvate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.2.45 | ? | x * 38000, His-tagged recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.2.45 | tHBP-HA | - |
Pseudomonas fluorescens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.45 | 40 | - |
at a fixed pH of 7.0 | Pseudomonas fluorescens |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.45 | 25 | 50 | - |
Pseudomonas fluorescens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.45 | 30 | 40 | after 30 min incubation, the decreases of the activity are 64% at 40°C and 24% at 35°C, while at 30°C, the activity remains unchanged. However, at 30°C, the decreases of the activity are 24% after 5 h and 54% after 24 h | Pseudomonas fluorescens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.45 | 7 | - |
- |
Pseudomonas fluorescens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.45 | 5.5 | 8.5 | - |
Pseudomonas fluorescens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
