EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.21 | expressed in Escherichia coli BL21(DE3) cells | Treponema denticola |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.1.1.21 | hanging drop vapor diffusion method, using 14-16% (w/v) PEG 1000, 100 mM MgCl2, and 100 mM imidazole (pH 8.0) | Treponema denticola |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.21 | T40N | the specific actzivity of the mutant is 20000fold lower than that of wild type enzyme | Treponema denticola |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.21 | 0.0038 | - |
4-carboxy-5-aminoimidazole ribonucleotide | 50 mM Tris-HCl (pH 8.0), at 10°C | Treponema denticola | |
4.1.1.21 | 0.009 | - |
4-carboxy-5-aminoimidazole ribonucleotide | 50 mM Tris-HCl (pH 8.0), at 30°C | Treponema denticola | |
4.1.1.21 | 0.06 | - |
5-aminoimidazole ribonucleotide | 50 mM Tris-HCl (pH 8.0), at 10°C | Treponema denticola | |
4.1.1.21 | 13 | - |
CO2 | 50 mM Tris-HCl (pH 8.0), at 10°C | Treponema denticola |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.21 | 17189 | - |
8 * 17189, calculated from amino acid sequence | Treponema denticola |
4.1.1.21 | 17195 | - |
8 * 17195, ESI-MS | Treponema denticola |
4.1.1.21 | 184000 | - |
gel filtration | Treponema denticola |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.21 | Treponema denticola | Q73PV9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.21 | streptomycin precipitation, ammonium sulfate precipitation, phenyl Sepharose 6 column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration | Treponema denticola |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.21 | 4-carboxy-5-aminoimidazole ribonucleotide + H+ | - |
Treponema denticola | 5-aminoimidazole ribonucleotide + CO2 | - |
r | |
4.1.1.21 | 5-aminoimidazole ribonucleotide + CO2 | - |
Treponema denticola | 4-carboxy-5-aminoimidazole ribonucleotide + H+ | - |
r | |
4.1.1.21 | additional information | the enzyme does not bind N5-carboxy-4-carboxy-5-aminoimidazole ribonucleotide | Treponema denticola | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.21 | homooctamer | 8 * 17189, calculated from amino acid sequence | Treponema denticola |
4.1.1.21 | homooctamer | 8 * 17195, ESI-MS | Treponema denticola |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.21 | AIR carboxylase | - |
Treponema denticola |
4.1.1.21 | class II PurE | - |
Treponema denticola |
4.1.1.21 | PurE | - |
Treponema denticola |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.21 | 16 | - |
4-carboxy-5-aminoimidazole ribonucleotide | 50 mM Tris-HCl (pH 8.0), at 10°C | Treponema denticola | |
4.1.1.21 | 65 | - |
4-carboxy-5-aminoimidazole ribonucleotide | 50 mM Tris-HCl (pH 8.0), at 30°C | Treponema denticola | |
4.1.1.21 | 77 | - |
CO2 | 50 mM Tris-HCl (pH 8.0), at 30°C | Treponema denticola |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.1.1.21 | Treponema denticola | calculated from amino acid sequence | - |
7.7 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.21 | 6.4 | - |
5-aminoimidazole ribonucleotide | 50 mM Tris-HCl (pH 8.0), at 10°C | Treponema denticola | |
4.1.1.21 | 33 | - |
4-carboxy-5-aminoimidazole ribonucleotide | 50 mM Tris-HCl (pH 8.0), at 30°C | Treponema denticola |