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Literature summary extracted from

  • Tranchimand, S.; Starks, C.M.; Mathews, I.I.; Hockings, S.C.; Kappock, T.J.
    Treponema denticola PurE is a bacterial AIR carboxylase (2011), Biochemistry, 50, 4623-4637.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.21 expressed in Escherichia coli BL21(DE3) cells Treponema denticola

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.21 hanging drop vapor diffusion method, using 14-16% (w/v) PEG 1000, 100 mM MgCl2, and 100 mM imidazole (pH 8.0) Treponema denticola

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.21 T40N the specific actzivity of the mutant is 20000fold lower than that of wild type enzyme Treponema denticola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.21 0.0038
-
4-carboxy-5-aminoimidazole ribonucleotide 50 mM Tris-HCl (pH 8.0), at 10°C Treponema denticola
4.1.1.21 0.009
-
4-carboxy-5-aminoimidazole ribonucleotide 50 mM Tris-HCl (pH 8.0), at 30°C Treponema denticola
4.1.1.21 0.06
-
5-aminoimidazole ribonucleotide 50 mM Tris-HCl (pH 8.0), at 10°C Treponema denticola
4.1.1.21 13
-
CO2 50 mM Tris-HCl (pH 8.0), at 10°C Treponema denticola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.21 17189
-
8 * 17189, calculated from amino acid sequence Treponema denticola
4.1.1.21 17195
-
8 * 17195, ESI-MS Treponema denticola
4.1.1.21 184000
-
gel filtration Treponema denticola

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.21 Treponema denticola Q73PV9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.21 streptomycin precipitation, ammonium sulfate precipitation, phenyl Sepharose 6 column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration Treponema denticola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.21 4-carboxy-5-aminoimidazole ribonucleotide + H+
-
Treponema denticola 5-aminoimidazole ribonucleotide + CO2
-
r
4.1.1.21 5-aminoimidazole ribonucleotide + CO2
-
Treponema denticola 4-carboxy-5-aminoimidazole ribonucleotide + H+
-
r
4.1.1.21 additional information the enzyme does not bind N5-carboxy-4-carboxy-5-aminoimidazole ribonucleotide Treponema denticola ?
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.21 homooctamer 8 * 17189, calculated from amino acid sequence Treponema denticola
4.1.1.21 homooctamer 8 * 17195, ESI-MS Treponema denticola

Synonyms

EC Number Synonyms Comment Organism
4.1.1.21 AIR carboxylase
-
Treponema denticola
4.1.1.21 class II PurE
-
Treponema denticola
4.1.1.21 PurE
-
Treponema denticola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.21 16
-
4-carboxy-5-aminoimidazole ribonucleotide 50 mM Tris-HCl (pH 8.0), at 10°C Treponema denticola
4.1.1.21 65
-
4-carboxy-5-aminoimidazole ribonucleotide 50 mM Tris-HCl (pH 8.0), at 30°C Treponema denticola
4.1.1.21 77
-
CO2 50 mM Tris-HCl (pH 8.0), at 30°C Treponema denticola

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.21 Treponema denticola calculated from amino acid sequence
-
7.7

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.21 6.4
-
5-aminoimidazole ribonucleotide 50 mM Tris-HCl (pH 8.0), at 10°C Treponema denticola
4.1.1.21 33
-
4-carboxy-5-aminoimidazole ribonucleotide 50 mM Tris-HCl (pH 8.0), at 30°C Treponema denticola