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Literature summary extracted from

  • Baker, P.; Carere, J.; Seah, S.Y.
    Probing the molecular basis of substrate specificity, stereospecificity, and catalysis in the class II pyruvate aldolase, BphI (2011), Biochemistry, 50, 3559-3569.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.39 expressed in Escherichia coli BL21(lambdaDE3) Paraburkholderia xenovorans
4.1.3.43 bphI previous to mutagenesis cloned into plasmid pBTL4-T7, Escherichia coli Paraburkholderia xenovorans

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.39 H20A decrease in kcat/Km value Paraburkholderia xenovorans
4.1.3.39 H20S decrease in kcat/Km value Paraburkholderia xenovorans
4.1.3.39 L87A 3-fold reduction in kcat/Km Paraburkholderia xenovorans
4.1.3.39 L87A/L89A improved specificity for aldehydes at least four carbons in length Paraburkholderia xenovorans
4.1.3.39 L89A kcat/Km similar to wild type Paraburkholderia xenovorans
4.1.3.39 R16A no detectable activity Paraburkholderia xenovorans
4.1.3.39 R16K increased Km value for 4-hydroxy-2-oxopentanoate Paraburkholderia xenovorans
4.1.3.39 Y290F lower kcat value Paraburkholderia xenovorans
4.1.3.39 Y290S lower kcat value Paraburkholderia xenovorans
4.1.3.43 H20A mutant generated by site-directed mutagenesis, pH profiles show the dependence of enzyme activity on hydroxide concentration Paraburkholderia xenovorans
4.1.3.43 H20S mutant generated by site-directed mutagenesis, pH profiles show the dependence of enzyme activity on hydroxide concentration Paraburkholderia xenovorans
4.1.3.43 L87A mutant generated by site-directed mutagenesis, variant exhibits a 40fold preference for propionaldehyde over acetaldehyde in contrast to wild type with similar specificities for acetaldehyde and propionaldehyde Paraburkholderia xenovorans
4.1.3.43 L89A mutant generated by site-directed mutagenesis, specificity constant of this variant in the aldol addition reaction using pentaldehyde is increased approx. 50fold, making it more catalytically efficient for pentaldehyde utilization compared to the wild-type utilization of the natural substrate, acetaldehyde Paraburkholderia xenovorans
4.1.3.43 R16A mutant generated by site-directed mutagenesis, no detectable aldol cleavage and pyruvate R-proton exchange, supporting the role of Arg-16 in stabilizing a pyruvate enolate intermediate Paraburkholderia xenovorans
4.1.3.43 Y290F mutant generated by site-directed mutagenesis, mutation results in a loss of stereochemical control as the variant is able to utilize substrates with R and S configurations at C4 with similar kinetic parameters Paraburkholderia xenovorans
4.1.3.43 Y290S mutant generated by site-directed mutagenesis, mutation results in a loss of stereochemical control as the variant is able to utilize substrates with R and S configurations at C4 with similar kinetic parameters Paraburkholderia xenovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.39 0.012
-
 (4R)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.013
-
 (4S)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.013
-
 (4R)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.013
-
 (4S)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.089
-
 (4S)-4-hydroxy-2-oxopentanoate wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.222
-
 (4S)-4-hydroxy-2-oxopentanoate H20A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.228
-
 (4S)-4-hydroxy-2-oxopentanoate H20S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 11
-
 pyruvate aldol addition reaction with propionaldehyde, L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 13
-
 pyruvate aldol addition reaction with propionaldehyde, wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 16.8
-
 propionaldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 20.2
-
 pyruvate aldol addition reaction with propionaldehyde, L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 24.8
-
 pentaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 33.3
-
 pentaldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 40.2
-
 propionaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 49.2
-
 Butyraldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 64.2
-
 acetaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 80.5
-
 acetaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 136
-
 propionaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 289
-
 Butyraldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 323
-
 Butyraldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 additional information
-
 additional information value not detectable for R16A variant of BphI Paraburkholderia xenovorans
4.1.3.43 0.012
-
 (4R)-4-hydroxy-2-oxopentanoate Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.013
-
 (4S)-4-hydroxy-2-oxopentanoate Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.013
-
 (4R)-4-hydroxy-2-oxopentanoate Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.013
-
 (4S)-4-hydroxy-2-oxopentanoate Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.089
-
 (4S)-4-hydroxy-2-oxopentanoate wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.222
-
 (4S)-4-hydroxy-2-oxopentanoate H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.228
-
 (4S)-4-hydroxy-2-oxopentanoate H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 11
-
 pyruvate L89A variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 13
-
 pyruvate wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 16.8
-
 propionaldehyde L87A variant of BphI, app. Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 20.2
-
 pyruvate L87A variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 24.8
-
 pentaldehyde L89A variant of BphI, app. Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 33.3
-
 pentaldehyde L87A/L89A variant of BphI, app. Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 40.2
-
 propionaldehyde L89A variant of BphI, app. Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 49.2
-
 Butyraldehyde L89A variant of BphI, app. Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 64.2
-
 acetaldehyde wild-type BphI, app. Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 80.5
-
 acetaldehyde L89A variant of BphI, app. Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 136
-
 propionaldehyde wild-type BphI, app. Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 289
-
 Butyraldehyde L87A/L89A variant of BphI, app. Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 323
-
 Butyraldehyde L87A variant of BphI, app. Km-value, pH 8.0, 25°C Paraburkholderia xenovorans

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.39 Paraburkholderia xenovorans
-
-
-
4.1.3.43 Paraburkholderia xenovorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.39
-
 Paraburkholderia xenovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.39 (4R)-4-hydroxy-2-oxopentanoate
-
 Paraburkholderia xenovorans acetaldehyde + pyruvate
-
 r
4.1.3.39 (4S)-4-hydroxy-2-oxopentanoate
-
 Paraburkholderia xenovorans acetaldehyde + pyruvate
-
 r
4.1.3.39 butyraldehyde + pyruvate
-
 Paraburkholderia xenovorans 4-hydroxy-2-oxoheptanoate
-
 r
4.1.3.39 hexaldehyde + pyruvate
-
 Paraburkholderia xenovorans 4-hydroxy-2-oxononanoate
-
 r
4.1.3.39 pentaldehyde + pyruvate
-
 Paraburkholderia xenovorans 4-hydroxy-2-oxooctanoate
-
 r
4.1.3.39 propionaldehyde + pyruvate
-
 Paraburkholderia xenovorans 4-hydroxy-2-oxohexanoate
-
 r
4.1.3.43 (4R)-4-hydroxy-2-oxopentanoate
-
 Paraburkholderia xenovorans pyruvate + acetaldehyde
-
 ?
4.1.3.43 (4S)-4-hydroxy-2-oxopentanoate
-
 Paraburkholderia xenovorans pyruvate + acetaldehyde
-
 ?
4.1.3.43 pyruvate + acetaldehyde
-
 Paraburkholderia xenovorans (4R)-4-hydroxy-2-oxopentanoate
-
 ?
4.1.3.43 pyruvate + acetaldehyde
-
 Paraburkholderia xenovorans (4S)-4-hydroxy-2-oxopentanoate
-
 ?
4.1.3.43 pyruvate + butyraldehyde
-
 Paraburkholderia xenovorans ?
-
 ?
4.1.3.43 pyruvate + hexaldehyde
-
 Paraburkholderia xenovorans ?
-
 ?
4.1.3.43 pyruvate + pentaldehyde
-
 Paraburkholderia xenovorans ?
-
 ?
4.1.3.43 pyruvate + propionaldehyde reaction catalyzed by BphI variants Y290F and Y290S Paraburkholderia xenovorans (4R)-4-hydroxy-2-oxohexanoate
-
 ?
4.1.3.43 pyruvate + propionaldehyde
-
 Paraburkholderia xenovorans (4S)-4-hydroxy-2-oxohexanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.3.39 class II pyruvate aldolase BphI
-
 Paraburkholderia xenovorans
4.1.3.43 BphI
-
 Paraburkholderia xenovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.39 0.089
-
 (4S)-4-hydroxy-2-oxopentanoate H20S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.099
-
 (4S)-4-hydroxy-2-oxopentanoate H20A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.13
-
 pentaldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.131
-
 (4R)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.132
-
 (4S)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.17
-
 (4R)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.171
-
 (4S)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.26
-
 Butyraldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.29
-
 propionaldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.3
-
 Butyraldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.32
-
 pyruvate aldol addition reaction with propionaldehyde, L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.58
-
 pentaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.63
-
 propionaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.64
-
 pyruvate aldol addition reaction with propionaldehyde, L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.64
-
 Butyraldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.86
-
 acetaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.94
-
 acetaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 1.2
-
 pyruvate aldol addition reaction with propionaldehyde, wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 1.79
-
 propionaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 4.07
-
 (4S)-4-hydroxy-2-oxopentanoate wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 additional information
-
 additional information value not detectable for R16A variant of BphI Paraburkholderia xenovorans
4.1.3.43 0.089
-
 (4S)-4-hydroxy-2-oxopentanoate H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.099
-
 (4S)-4-hydroxy-2-oxopentanoate H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.13
-
 pentaldehyde L87A/L89A variant of BphI, app. kcat-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.131
-
 (4R)-4-hydroxy-2-oxopentanoate Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.132
-
 (4S)-4-hydroxy-2-oxopentanoate Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.17
-
 (4R)-4-hydroxy-2-oxopentanoate Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.171
-
 (4S)-4-hydroxy-2-oxopentanoate Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.26
-
 Butyraldehyde L87A variant of BphI, app. kcat-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.29
-
 propionaldehyde L87A variant of BphI, app. kcat-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.3
-
 Butyraldehyde L87A/L89A variant of BphI, app. kcat-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.32
-
 pyruvate L87A variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.58
-
 pentaldehyde L89A variant of BphI, app. kcat-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.63
-
 propionaldehyde L89A variant of BphI, app. kcat-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.64
-
 Butyraldehyde L89A variant of BphI, app. kcat-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.64
-
 pyruvate L89A variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.86
-
 acetaldehyde wild-type BphI, app. kcat-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.94
-
 acetaldehyde L89A variant of BphI, app. kcat-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 1.2
-
 pyruvate wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 1.79
-
 propionaldehyde wild-type BphI, app. kcat-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 4.07
-
 (4S)-4-hydroxy-2-oxopentanoate wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.39 8.5
-
-
 Paraburkholderia xenovorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.3.39 6.5 8.5
-
 Paraburkholderia xenovorans

General Information

EC Number General Information Comment Organism
4.1.3.39 physiological function polychlorinated biphenyl degradation pathway Paraburkholderia xenovorans
4.1.3.43 metabolism enzyme found in the polychlorinated biphenyls (PCBs) degradation pathway Paraburkholderia xenovorans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.3.39 0.00001
-
 hexaldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.00001
-
 hexaldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.00001
-
 hexaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.00013
-
 hexaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.00033
-
 pentaldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.00042
-
 acetaldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.00047
-
 pentaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.00049
-
 acetaldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.00079
-
 Butyraldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.00104
-
 Butyraldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.00219
-
 Butyraldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.0038
-
 pentaldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.00673
-
 propionaldehyde L87A/L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.0117
-
 acetaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.012
-
 (4S)-4-hydroxy-2-oxopentanoate R16K mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.0129
-
 Butyraldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.0132
-
 propionaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.0134
-
 acetaldehyde wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.0156
-
 propionaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.0159
-
 pyruvate aldol addition reaction with propionaldehyde, L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.0175
-
 propionaldehyde L87A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.0233
-
 pentaldehyde L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.0587
-
 pyruvate aldol addition reaction with propionaldehyde, L89A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.0915
-
 pyruvate aldol addition reaction with propionaldehyde, wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.34
-
 (4S)-4-hydroxy-2-oxopentanoate H20S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 0.45
-
 (4S)-4-hydroxy-2-oxopentanoate H20A mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 10.4
-
 (4R)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 12.6
-
 (4S)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 13.4
-
 (4S)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 14.1
-
 (4R)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.39 44.7
-
 (4S)-4-hydroxy-2-oxopentanoate wild type protein, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 additional information
-
 additional information value not detectable for R16A variant of BphI Paraburkholderia xenovorans
4.1.3.43 0.00001
-
 hexaldehyde L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.00001
-
 hexaldehyde wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.0001
-
 hexaldehyde L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.00013
-
 hexaldehyde L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.00033
-
 pentaldehyde L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.00042
-
 acetaldehyde L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.00047
-
 pentaldehyde wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.00049
-
 acetaldehyde L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.00079
-
 Butyraldehyde L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.00104
-
 Butyraldehyde L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.0022
-
 Butyraldehyde wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.0038
-
 pentaldehyde L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.00673
-
 propionaldehyde L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.0117
-
 acetaldehyde L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.012
-
 (4S)-4-hydroxy-2-oxopentanoate R16K variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.0129
-
 Butyraldehyde L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.0132
-
 propionaldehyde wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.0134
-
 acetaldehyde wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.0156
-
 propionaldehyde L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.0159
-
 pyruvate L87A variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.0175
-
 propionaldehyde L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.0233
-
 pentaldehyde L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.0587
-
 pyruvate L89A variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.0915
-
 pyruvate wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.34
-
 (4S)-4-hydroxy-2-oxopentanoate H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 0.45
-
 (4S)-4-hydroxy-2-oxopentanoate H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 10.4
-
 (4R)-4-hydroxy-2-oxopentanoate Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 12.6
-
 (4S)-4-hydroxy-2-oxopentanoate Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 13.4
-
 (4S)-4-hydroxy-2-oxopentanoate Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 14.1
-
 (4R)-4-hydroxy-2-oxopentanoate Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans
4.1.3.43 44.7
-
 (4S)-4-hydroxy-2-oxopentanoate wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C Paraburkholderia xenovorans