EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.2.1 | expressed in Escherichia coli Rosetta(DE3) cells | Trypanosoma brucei brucei |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.2.1 | hanging drop vapor diffusion method, using 100 mM Bis-Tris (pH 6.5), 200 mM ammonium sulfate, and 22% (w/v) polyethylene glycol monomethyl ether 3350 | Trypanosoma brucei brucei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.1 | 2'-deoxyadenosine | - |
Trypanosoma brucei brucei | |
3.2.2.1 | 3'-deoxyadenosine | - |
Trypanosoma brucei brucei | |
3.2.2.1 | 8-aza-immucillin-H | nanomolar inhibitor | Trypanosoma brucei brucei | |
3.2.2.1 | immucillin-A | nanomolar inhibitor | Trypanosoma brucei brucei | |
3.2.2.1 | immucillin-H | nanomolar inhibitor | Trypanosoma brucei brucei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.1 | 0.0006 | - |
purine riboside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.0008 | - |
2-aminopurine riboside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.001 | - |
adenosine | Km less than 0.001 mM, at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.0011 | - |
4-nitrophenyl beta-D-ribofuranoside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.0019 | - |
Inosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.0022 | - |
3-deazaadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.0036 | - |
ethenoadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.0045 | - |
guanosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.24 | - |
5'-deoxyadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.271 | - |
cytidine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.28 | - |
Xanthosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 1.451 | - |
uridine | at pH 7.0 and 35°C | Trypanosoma brucei brucei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.1 | guanosine + H2O | Trypanosoma brucei brucei | - |
D-ribose + guanine | - |
? | |
3.2.2.1 | inosine + H2O | Trypanosoma brucei brucei | - |
D-ribose + hypoxanthine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.1 | Trypanosoma brucei brucei | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.1 | Ni-NTA resin column chromatography and Superdex 200 gel filtration | Trypanosoma brucei brucei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.1 | 2-aminopurine riboside + H2O | - |
Trypanosoma brucei brucei | 2-aminopurine + D-ribose | - |
? | |
3.2.2.1 | 3-deazaadenosine + H2O | - |
Trypanosoma brucei brucei | D-ribose + 3-deazaadenine | - |
? | |
3.2.2.1 | 4-nitrophenyl beta-D-ribofuranoside + H2O | - |
Trypanosoma brucei brucei | 4-nitrophenol + beta-D-ribofuranose | - |
? | |
3.2.2.1 | 5'-deoxyadenosine + H2O | - |
Trypanosoma brucei brucei | 5-deoxy-D-ribose + adenine | - |
? | |
3.2.2.1 | 7-methylguanosine + H2O | - |
Trypanosoma brucei brucei | ? | - |
? | |
3.2.2.1 | adenosine + H2O | - |
Trypanosoma brucei brucei | D-ribose + adenine | - |
? | |
3.2.2.1 | cytidine + H2O | - |
Trypanosoma brucei brucei | D-ribose + cytosine | - |
? | |
3.2.2.1 | ethenoadenosine + H2O | - |
Trypanosoma brucei brucei | D-ribose + ethenoadenine | - |
? | |
3.2.2.1 | guanosine + H2O | - |
Trypanosoma brucei brucei | D-ribose + guanine | - |
? | |
3.2.2.1 | guanosine + H2O | preferred substrate | Trypanosoma brucei brucei | D-ribose + guanine | - |
? | |
3.2.2.1 | inosine + H2O | - |
Trypanosoma brucei brucei | D-ribose + hypoxanthine | - |
? | |
3.2.2.1 | inosine + H2O | preferred substrate | Trypanosoma brucei brucei | D-ribose + hypoxanthine | - |
? | |
3.2.2.1 | purine riboside + H2O | - |
Trypanosoma brucei brucei | purine + D-ribose | - |
? | |
3.2.2.1 | uridine + H2O | - |
Trypanosoma brucei brucei | D-ribose + uracil | - |
? | |
3.2.2.1 | xanthosine + H2O | - |
Trypanosoma brucei brucei | D-ribose + xanthine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.2.1 | homotetramer | x-ray crystallography | Trypanosoma brucei brucei |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.2.1 | 6-oxopurine nucleosidase | - |
Trypanosoma brucei brucei |
3.2.2.1 | IG-NH | - |
Trypanosoma brucei brucei |
3.2.2.1 | inosine-guanosine-specific nucleoside hydrolase | - |
Trypanosoma brucei brucei |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.1 | 0.0038 | - |
uridine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.009 | - |
3-deazaadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.03 | - |
4-nitrophenyl beta-D-ribofuranoside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.078 | - |
5'-deoxyadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.237 | - |
adenosine | Km less than 0.001 mM, at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.248 | - |
purine riboside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.52 | - |
cytidine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.57 | - |
2-aminopurine riboside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 1.3 | - |
guanosine | at pH 7.0 and 5°C | Trypanosoma brucei brucei | |
3.2.2.1 | 2 | 8 | Inosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 5.2 | - |
7-methylguanosine | at pH 7.0 and 5°C | Trypanosoma brucei brucei | |
3.2.2.1 | 6.2 | - |
Xanthosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 6.9 | - |
ethenoadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 37.6 | - |
guanosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.1 | 0.000009 | - |
immucillin-A | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.000015 | - |
8-aza-immucillin-H | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.000044 | - |
immucillin-H | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.18 | - |
3'-deoxyadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.182 | - |
2'-deoxyadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.1 | 0.0026 | - |
uridine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 0.33 | - |
5'-deoxyadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 1.9 | - |
cytidine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 22 | - |
Xanthosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 27 | - |
4-nitrophenyl beta-D-ribofuranoside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 41 | - |
3-deazaadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 240 | - |
adenosine | kcat_Km above 240/sec*mM, at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 380 | - |
purine riboside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 700 | - |
2-aminopurine riboside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 1900 | - |
ethenoadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 8300 | - |
guanosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
3.2.2.1 | 15000 | - |
Inosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei |