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Literature summary extracted from

  • Satoh, A.; Konishi, S.; Tamura, H.; Stickland, H.G.; Whitney, H.M.; Smith, A.G.; Matsumura, H.; Inoue, T.
    Substrate-induced closing of the active site revealed by the crystal structure of pantothenate synthetase from Staphylococcus aureus (2010), Biochemistry, 49, 6400-6410.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.1 expressed in Escherichia coli as a His-tagged fusion protein Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.2.1 structures of the apoenzyme and the reaction intermediate complex are determined by X-ray crystallography to resolutions of 2.5 A and 1.85 A, respectively. Structural analysis indicate that the apoenzyme adopts an open and relatively mobile structure, while the complex structure is closed and entirely rigid. In the complex structure, pantothenate synthetase and acetate are bound in the active site. Acetate might mimic the substrate beta-alanine. Therefore, the complex structure might represent a catalytic state poised for in-line nucleophilic attack on pantothenate synthetase Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.1 Mg2+
-
 Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.1 Staphylococcus aureus Q2FV22
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.1 using Ni-NTA chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.1 ATP + (R)-pantoate + beta-alanine
-
 Staphylococcus aureus AMP + diphosphate + (R)-pantothenate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.1 Pantothenate synthetase
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.1 30
-
-
 Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.1 1.5
-
 beta-Alanine co-substrate: (R)-pantoate, pH 8.0, 30°C Staphylococcus aureus
6.3.2.1 1.5
-
 (R)-pantoate co-substrate: beta-alanine, pH 8.0, 30°C Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.1 8.5
-
-
 Staphylococcus aureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.1 8 9
-
 Staphylococcus aureus