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Literature summary extracted from

  • Melançon, C.E. 3rd.; Hong, L.; White, J.A.; Liu, Y.N.; Liu, H.W.
    Characterization of TDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase from the D-mycaminose biosynthetic pathway of Streptomyces fradiae: in vitro activity and substrate specificity studies (2007), Biochemistry, 46, 577-590.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.89 expression in Escherichia coli Streptomyces fradiae
5.3.2.4 expression in Escherichia coli Streptomyces fradiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.2.4 0.027
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose pH 7.5, 25°C Streptomyces fradiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.2.4 18806
-
 2 * 18806, calculated from sequence Streptomyces fradiae
5.3.2.4 19000
-
 2 * 19000, SDS-PAGE Streptomyces fradiae
5.3.2.4 31000
-
 gel filtration Streptomyces fradiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.89 dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate Streptomyces fradiae the enzyme is involved in biosynthesis of D-mycaminose dTDP-3-dehydro-6-deoxy-alpha-D-glucopyranose + L-glutamate
-
 r
5.3.2.4 dTDP-4-dehydro-6-deoxy-alpha-D-glucose Streptomyces fradiae the enzyme is involved in the D-mycaminose biosynthetic pathway dTDP-3-dehydro-6-deoxy-alpha-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.89 Streptomyces fradiae
-
-
-
5.3.2.4 Streptomyces fradiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.89
-
 Streptomyces fradiae
5.3.2.4
-
 Streptomyces fradiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.89 CDP-3-dehydro-6-deoxy-alpha-D-glucose + L-glutamate
-
 Streptomyces fradiae CDP-3-amino-3,6-dideoxy-alpha-D-glucose + 2-oxoglutarate
-
 ?
2.6.1.89 dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate the enzyme is involved in biosynthesis of D-mycaminose Streptomyces fradiae dTDP-3-dehydro-6-deoxy-alpha-D-glucopyranose + L-glutamate
-
 r
2.6.1.89 dTDP-3-dehydro-2,6-dideoxy-alpha-D-glucose + L-glutamate
-
 Streptomyces fradiae TDP-3-amino-2,3,6-trideoxy-alpha-D-glucose + 2-oxoglutarate
-
 ?
2.6.1.89 dTDP-3-dehydro-6-deoxy-alpha-D-glucopyranose + L-glutamate
-
 Streptomyces fradiae dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate no formation of TDP-4-oxo-6-deoxy-alpha-D-glucose r
2.6.1.89 additional information no activity with TDP-4-keto-6-deoxy-D-glucose Streptomyces fradiae ?
-
 ?
5.3.2.4 CDP-4-dehydro-6-deoxy-alpha-D-glucose efficiency of conversion is low, requiring a high concentration of enzyme and a long incubation time to achieve significant turnover Streptomyces fradiae CDP-3-dehydro-6-deoxy-alpha-D-glucose
-
 ?
5.3.2.4 dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose efficiency is comparable to that with dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose Streptomyces fradiae dTDP-3-dehydro-2,6-dideoxy-alpha-D-glucose
-
 ?
5.3.2.4 dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose
-
 Streptomyces fradiae dTDP-3-dehydro-6-deoxy-alpha-D-glucopyranose
-
 ?
5.3.2.4 dTDP-4-dehydro-6-deoxy-alpha-D-glucose the enzyme is involved in the D-mycaminose biosynthetic pathway Streptomyces fradiae dTDP-3-dehydro-6-deoxy-alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.2.4 dimer 2 * 19000, SDS-PAGE Streptomyces fradiae
5.3.2.4 dimer 2 * 18806, calculated from sequence Streptomyces fradiae

Synonyms

EC Number Synonyms Comment Organism
2.6.1.89 TDP-3-dehydro-6-deoxy-D-glucose 3-aminotransferase
-
 Streptomyces fradiae
2.6.1.89 TDP-3-keto-6-deoxy-D-glucose 3-aminotransferase
-
 Streptomyces fradiae
2.6.1.89 TylB
-
 Streptomyces fradiae
5.3.2.4 TDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase
-
 Streptomyces fradiae
5.3.2.4 Tyl1a
-
 Streptomyces fradiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.89 25
-
 assay at Streptomyces fradiae
5.3.2.4 25
-
 assay at Streptomyces fradiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.2.4 0.00025
-
 CDP-4-dehydro-6-deoxy-alpha-D-glucose pH 7.5, 25°C Streptomyces fradiae
5.3.2.4 0.1
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose pH 7.5, 25°C Streptomyces fradiae
5.3.2.4 0.13
-
 dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose pH 7.5, 25°C Streptomyces fradiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.89 7.5
-
 assay Streptomyces fradiae
5.3.2.4 7.5
-
 assay Streptomyces fradiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.89 pyridoxal 5'-phosphate a pyridoxal-phosphate protein Streptomyces fradiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.2.4 3.7
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose pH 7.5, 25°C Streptomyces fradiae