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Literature summary extracted from

  • Thomas, J.W.; Lemieux, L.J.; Alben, J.O.; Gennis, R.B.
    Site-directed mutagenesis of highly conserved residues in helix VIII of subunit I of the cytochrome bo ubiquinol oxidase from Escherichia coli: an amphipathic transmembrane helix that may be important in conveying protons to the binuclear center (1993), Biochemistry, 32, 11173-11180.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.3 expressed in Escherichia coli RG129 cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.3 K362L catalytically inactive Escherichia coli
7.1.1.3 K362M catalytically inactive Escherichia coli
7.1.1.3 K362Q catalytically inactive Escherichia coli
7.1.1.3 M353A the mutant shows substantial activity Escherichia coli
7.1.1.3 P358A the mutant shows substantial activity Escherichia coli
7.1.1.3 T352A catalytically inactive Escherichia coli
7.1.1.3 T352N the mutant shows substantial activity Escherichia coli
7.1.1.3 T352S the mutant shows substantial activity Escherichia coli
7.1.1.3 T359A catalytically inactive Escherichia coli
7.1.1.3 T359S the mutant shows almost wild type activity Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.3 membrane
-
Escherichia coli 16020
-

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.3 Escherichia coli
-
-
-
7.1.1.3 Escherichia coli GL101
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.3 ubiquinol-1 + O2 + H+/in
-
Escherichia coli ubiquinone-1 + H2O + H+/out
-
?
7.1.1.3 ubiquinol-1 + O2 + H+/in
-
Escherichia coli GL101 ubiquinone-1 + H2O + H+/out
-
?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.3 cytochrome bo ubiquinol oxidase
-
Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.3 heme heme b562 Escherichia coli