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Literature summary extracted from

  • Yang, Q.; Liu, Y.; Huang, F.; He, Z.G.
    Physical and functional interaction between D-ribokinase and topoisomerase I has opposite effects on their respective activity in Mycobacterium smegmatis and Mycobacterium tuberculosis (2011), Arch. Biochem. Biophys., 512, 135-142.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.15 MtbTopA when increasing amounts of Mycobacterium smegmatis topoisomerase I are mixed together with ribokinase, the amount of phosphorylated D-ribose products steadily increases, indicating that Mycobacterium smegmatis topoisomerase I stimulates the kinase activity of ribokinase Mycobacterium tuberculosis
2.7.1.15 topoisomerase I when increasing amounts of MsTopA are mixed together with ribokinase, the amount of phosphorylated D-ribose products steadily increases, indicating that topoisomerase I stimulates the kinase activity of Ms4585 Mycolicibacterium smegmatis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.15 expressed in Escherichia coli Mycolicibacterium smegmatis
2.7.1.15 expressed in Escherichia coli Mycobacterium tuberculosis
5.6.2.1 gene MsTopA, co-expression with gene Ms4585, encoding a ribokinase, in Escherichia coli strain BL21. Interaction analysis using the two-hybrid system Mycolicibacterium smegmatis
5.6.2.1 gene MtbTopA, co-expression with gene Rv2436, encoding a ribokinase of Mycobacterium tuberculosis, in Escherichia coli strain BL21. Interaction analysis using the two-hybrid system Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.15 A13/103/116V mutant shows no interaction with topoisomerase I. Mutant only partially retains its ability to inhibit the activity of topoisomerase I even at high protein concentrations. Topoisomerase I does not significantly stimulate the activity of the mutant protein Mycolicibacterium smegmatis
2.7.1.15 S11N/A14V mutant shows no interaction with topoisomerase I Mycobacterium tuberculosis
2.7.1.15 S11N/A14V mutant shows no interaction with topoisomerase I. Mutant only partially retains its ability to inhibit the activity of topoisomerase I even at high protein concentrations. Topoisomerase I does not significantly stimulate the activity of the mutant protein Mycolicibacterium smegmatis
5.6.2.1 additional information the His6 tagged-MtbTopA protein is immobilized on an NTA chip. When an increasing amount of GST tagged-Rv2436 protein pass over the chip, they cause a substantial response, overview Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.1 Ms4585 inhibits the function of MsTopA in relaxing supercoiled DNA. The ribokinase, encoded by gene Ms4585, acts as a netaive regulator of TopA activity Mycolicibacterium smegmatis
5.6.2.1 Rv2436 inhibits the TopA activity on supercoiled DNA. The ribokinase, encoded by gene Rv2436, acts as a netaive regulator of TopA activity Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.15 additional information Mycolicibacterium smegmatis mycobacterial topoisomerase I (TopA) is found to physically interact with ribokinase both in vitro and in vivo. The interaction between ribokinase and TopA has opposite effects on their respective function. While the interaction between the two proteins inhibits the ability of TopA to relax supercoiled DNA, it stimulates ribokinase activity ?
-
 ?
2.7.1.15 additional information Mycobacterium tuberculosis mycobacterial topoisomerase I (TopA) is found to physically interact with ribokinase both in vitro and in vivo. The interaction between ribokinase and topoisomerase I has opposite effects on their respective function. While the interaction between the two proteins inhibits the ability of topoisomerase I to relax supercoiled DNA, it stimulates ribokinase activity ?
-
 ?
5.6.2.1 additional information Mycolicibacterium smegmatis MsTopA stimulates the phosphorylating activity of Ms3759 on D-ribose, and physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. Interaction analysis, overview ?
-
 ?
5.6.2.1 additional information Mycobacterium tuberculosis the mycobacterial topoisomerase I physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. Interaction analysis, overview ?
-
 ?
5.6.2.1 additional information Mycobacterium tuberculosis H37Rv the mycobacterial topoisomerase I physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. Interaction analysis, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.15 Mycobacterium tuberculosis
-
-
-
2.7.1.15 Mycolicibacterium smegmatis
-
-
-
5.6.2.1 Mycobacterium tuberculosis P9WG49 gene MtbTopA or Rv3646
-
5.6.2.1 Mycobacterium tuberculosis H37Rv P9WG49 gene MtbTopA or Rv3646
-
5.6.2.1 Mycolicibacterium smegmatis
-
 gene MsTopA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.15 ATP + D-ribose
-
 Mycolicibacterium smegmatis ADP + D-ribose 5-phosphate
-
 ?
2.7.1.15 ATP + D-ribose
-
 Mycobacterium tuberculosis ADP + D-ribose 5-phosphate
-
 ?
2.7.1.15 additional information mycobacterial topoisomerase I (TopA) is found to physically interact with ribokinase both in vitro and in vivo. The interaction between ribokinase and TopA has opposite effects on their respective function. While the interaction between the two proteins inhibits the ability of TopA to relax supercoiled DNA, it stimulates ribokinase activity Mycolicibacterium smegmatis ?
-
 ?
2.7.1.15 additional information mycobacterial topoisomerase I (TopA) is found to physically interact with ribokinase both in vitro and in vivo. The interaction between ribokinase and topoisomerase I has opposite effects on their respective function. While the interaction between the two proteins inhibits the ability of topoisomerase I to relax supercoiled DNA, it stimulates ribokinase activity Mycobacterium tuberculosis ?
-
 ?
5.6.2.1 additional information MsTopA stimulates the phosphorylating activity of Ms3759 on D-ribose, and physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. Interaction analysis, overview Mycolicibacterium smegmatis ?
-
 ?
5.6.2.1 additional information the mycobacterial topoisomerase I physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. Interaction analysis, overview Mycobacterium tuberculosis ?
-
 ?
5.6.2.1 additional information substrate is supercoiled plasmid DNA Mycolicibacterium smegmatis ?
-
 ?
5.6.2.1 additional information substrate is supercoiled plasmid DNA Mycobacterium tuberculosis ?
-
 ?
5.6.2.1 additional information the mycobacterial topoisomerase I physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. Interaction analysis, overview Mycobacterium tuberculosis H37Rv ?
-
 ?
5.6.2.1 additional information substrate is supercoiled plasmid DNA Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.15 D-ribokinase
-
 Mycolicibacterium smegmatis
2.7.1.15 D-ribokinase
-
 Mycobacterium tuberculosis
2.7.1.15 Ms4585
-
 Mycolicibacterium smegmatis
2.7.1.15 Rv2436
-
 Mycobacterium tuberculosis
5.6.2.1 TopA
-
 Mycolicibacterium smegmatis
5.6.2.1 TopA
-
 Mycobacterium tuberculosis
5.6.2.1 Topoisomerase I
-
 Mycolicibacterium smegmatis
5.6.2.1 Topoisomerase I
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.15 37
-
 assay at Mycolicibacterium smegmatis
2.7.1.15 37
-
 assay at Mycobacterium tuberculosis
5.6.2.1 37
-
 assay at Mycolicibacterium smegmatis
5.6.2.1 37
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.2.1 7.8
-
 assay at Mycolicibacterium smegmatis
5.6.2.1 7.8
-
 assay at Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.7.1.15 physiological function ribokinase inhibits the function of MsTopA in relaxing supercoiled DNA Mycolicibacterium smegmatis
2.7.1.15 physiological function ribokinase inhibits the function of topoisomerase I in relaxing supercoiled DNA Mycobacterium tuberculosis
5.6.2.1 metabolism the mycobacterial topoisomerase I physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. While the interaction between the two proteins inhibits the ability of TopA to relax supercoiled DNA, it stimulates ribokinase activity as a regulatory strategy for efficient utilization of D-ribose Mycolicibacterium smegmatis
5.6.2.1 metabolism the mycobacterial topoisomerase I physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. While the interaction between the two proteins inhibits the ability of TopA to relax supercoiled DNA, it stimulates ribokinase activity as a regulatory strategy for efficient utilization of D-ribose Mycobacterium tuberculosis