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Literature summary extracted from
- Engineering of a fungal beta-galactosidase to remove product inhibition by galactose (2010), Appl. Microbiol. Biotechnol., 87, 1773-1782.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.23 | expressed in Pichia pastoris as a His-tagged fusion protein | Aspergillus niger |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.23 | S259F/P261T/L262S/G263S | mutant shows slightly reduced affinity for substrate (Km: 3.76 mM), 8.5fold reduction in inhibition by galactose, maximal activity lower than wild-type, pH stability lower than wild-type | Aspergillus niger |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.23 | D-galactose | - |
Aspergillus niger |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.23 | 2.21 | - |
2-nitrophenyl beta-D-galactopyranoside | wild-type, pH 5.5, 42° | Aspergillus niger | |
| 3.2.1.23 | 3.76 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant S259F/P261T/L262S/G263S, pH 5.5, 42° | Aspergillus niger | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.23 | Aspergillus niger | B6HYI9 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.23 | using Ni-NTA chromatography | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.23 | 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Aspergillus niger | 2-nitrophenol + beta-D-galactose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.23 | beta-galactosidase | - |
Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 2.5 | - |
wild-type | Aspergillus niger |
| 3.2.1.23 | 4.5 | - |
mutant S259F/P261T/L262S/G263S | Aspergillus niger |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.23 | 0.76 | - |
D-galactose | wild-type, pH 5.5, 42° | Aspergillus niger | |
| 3.2.1.23 | 6.46 | - |
D-galactose | mutant S259F/P261T/L262S/G263S, pH 5.5, 42° | Aspergillus niger | |
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Release 2023.1 (January 2023)
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