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Literature summary extracted from

  • Gangoiti, J.; Santos, M.; Llama, M.J.; Serra, J.L.
    Production of chiral (R)-3-hydroxyoctanoic acid monomers, catalyzed by Pseudomonas fluorescens GK13 poly(3-hydroxyoctanoic acid) depolymerase (2010), Appl. Environ. Microbiol., 76, 3554-3560.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.76 additional information the activity of the pure enzyme is enhanced by the presence of detergents and organic solvents, and it is retained after treatment with an SDS-denaturing cocktail under both reducing and nonreducing conditions Pseudomonas fluorescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.76 extracellular
-
 Pseudomonas fluorescens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.76 26000
-
 x * 26000, SDS-PAGE Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.76 poly(3-hydroxyoctanoic acid) + H2O Pseudomonas fluorescens the dimeric ester of 3-hydroxyoctanoic acid is obtained as the main product of the soluble enzyme ?
-
 ?
3.1.1.76 poly(3-hydroxyoctanoic acid) + H2O Pseudomonas fluorescens GK13 the dimeric ester of 3-hydroxyoctanoic acid is obtained as the main product of the soluble enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.76 Pseudomonas fluorescens
-
-
-
3.1.1.76 Pseudomonas fluorescens GK13
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.76 development of a rapid and easy immobilization method of the enzyme, the extracellular enzyme is tightly adsorbed onto a commercially available polypropylene support with high yield and specificity. The immobilized enzyme catalyzes almost the complete hydrolysis of poly(3-hydroxyoctanoic acid) polymer to (R)-3-hydroxyoctanoic acid monomers Pseudomonas fluorescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.76 additional information strain GK13 is grown on mineral medium supplemented with poly(3-hydroxyoctanoic acid) as the sole carbon and energy source Pseudomonas fluorescens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.76 19.4
-
 substrate 4-nitrophenyl octanoate, pH 9.5, 40°C Pseudomonas fluorescens
3.1.1.76 76
-
 substrate poly(3-hydroxyoctanoic acid), pH 9.5, 40°C Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
3.1.1.76 the purified enzyme retains at least 75% of its original activity after 5 months of storage at -20 and -80°C, respectively Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.76 4-nitrophenyl octanoate + H2O
-
 Pseudomonas fluorescens octanoate + 4-nitrophenol
-
 ?
3.1.1.76 4-nitrophenyl octanoate + H2O
-
 Pseudomonas fluorescens GK13 octanoate + 4-nitrophenol
-
 ?
3.1.1.76 poly(3-hydroxyoctanoic acid) + H2O the dimeric ester of 3-hydroxyoctanoic acid is obtained as the main product of the soluble enzyme Pseudomonas fluorescens ?
-
 ?
3.1.1.76 poly(3-hydroxyoctanoic acid) + H2O the dimeric ester of 3-hydroxyoctanoic acid is obtained as the main product of the soluble enzyme Pseudomonas fluorescens GK13 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.76 ? x * 26000, SDS-PAGE Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.76 MCL-PHA depolymerase
-
 Pseudomonas fluorescens
3.1.1.76 medium-chain-length polyhydroxyalkanoate depolymerase
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.76 40
-
-
 Pseudomonas fluorescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.76 45
-
 relatively high stability at temperatures below 45°C Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.76 9.5
-
-
 Pseudomonas fluorescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.76 8 11 over 60% of maximal activity within this range Pseudomonas fluorescens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.76 Pseudomonas fluorescens peak 1, isoelectric focusing
-
 6.45
3.1.1.76 Pseudomonas fluorescens peak 2, isoelectric focusing
-
 6.95