Any feedback?
Literature summary extracted from
- Differential properties of Aspergillus niger tannase produced under solid-state and submerged fermentations (2011), Appl. Biochem. Biotechnol., 165, 382-395.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.20 | Ca2+ | 7% inhibition of tannase produced under submerged fermentation at 1 mM | Aspergillus niger |  |
| 3.1.1.20 | Cu2+ | 41% inhibition of tannase produced under solid-state fermentation at 1 mM | Aspergillus niger | |
| 3.1.1.20 | Fe2+ | 63% inhibition of tannase produced under submerged fermentation at 1 mM | Aspergillus niger | |
| 3.1.1.20 | K+ | - |
Aspergillus niger | |
| 3.1.1.20 | Mg2+ | - |
Aspergillus niger | |
| 3.1.1.20 | Mn2+ | - |
Aspergillus niger | |
| 3.1.1.20 | Zn2+ | - |
Aspergillus niger | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.20 | 0.49 | - |
Tannic acid | enzyme produced under submerged fermentation, at pH 7.0, 30°C | Aspergillus niger | |
| 3.1.1.20 | 0.78 | - |
methyl gallate | enzyme produced under solid-state fermentation, at pH 7.0, 30°C | Aspergillus niger | |
| 3.1.1.20 | 1.4 | - |
Tannic acid | enzyme produced under solid-state fermentation, at pH 7.0, 30°C | Aspergillus niger | |
| 3.1.1.20 | 7.41 | - |
methyl gallate | enzyme produced under submerged fermentation, at pH 7.0, 30°C | Aspergillus niger | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 102000 | - |
x * 102000, enzyme produced under solid-state fermentation, SDS-PAGE | Aspergillus niger |
| 3.1.1.20 | 105000 | - |
x * 105000, enzyme produced under submerged fermentation, SDS-PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.20 | tannic acid + H2O | Aspergillus niger | - |
gallic acid + D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.20 | Aspergillus niger | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | glycoprotein | - |
Aspergillus niger |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.20 | isoelectric focusing, High Q column chromatography, High Trap Q column chromatography, High S column chromatography, and Superdex G-200 gel filtration | Aspergillus niger |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 264.5 | - |
enzyme produced under solid-state fermentation, after 153fold purification, at pH 7.0, 30°C | Aspergillus niger |
| 3.1.1.20 | 2067 | - |
enzyme produced under submerged fermentation, after 477fold purification, at pH 7.0, 30°C | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.20 | methyl gallate + H2O | - |
Aspergillus niger | gallate + methanol | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Aspergillus niger | gallic acid + D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | ? | x * 102000, enzyme produced under solid-state fermentation, SDS-PAGE | Aspergillus niger |
| 3.1.1.20 | ? | x * 105000, enzyme produced under submerged fermentation, SDS-PAGE | Aspergillus niger |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | tannin acyl hydrolase | - |
Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 50 | 60 | enzyme produced under submerged fermentation | Aspergillus niger |
| 3.1.1.20 | 60 | - |
enzyme produced under solid-state fermentation | Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 60 | 70 | solid-state fermentation tannase and submerged fermentation tannase are stable at 60°C for 1 h incubation retaining around 90% of activity. Both purified tannases lose the activity at 80°C, nevertheless, the submerged fermentation tannase only loses 20% of activity at 70°C for 1 h incubation and solid-state fermentation tannase retaining 30% of activity at the same condition | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 4 | - |
a minor secondary peak at pH 4.0 is found in both enzymes produced under solid-state and submerged fermentations with 70% of tannase activation | Aspergillus niger |
| 3.1.1.20 | 6 | - |
pH optimum for both enzymes produced under solid-state and submerged fermentations | Aspergillus niger |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 5 | 7 | the stability pH is 6.0 at 4°C for 24 h for both purified tannases maintaining 100% of enzymatic activity. Submerged fermentation tannase supports 90-100% enzymatic activity at pH 5.0-7.0. The solid-state fermentation tannase at pH values of 5.0 and 6.0, supports 85-100% activity | Aspergillus niger |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
