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Literature summary extracted from

  • Ramos, E.L.; Mata-Gomez, M.A.; Rodriguez-Duran, L.V.; Belmares, R.E.; Rodriguez-Herrera, R.; Aguilar, C.N.
    Catalytic and thermodynamic properties of a tannase produced by Aspergillus niger GH1 grown on polyurethane foam (2011), Appl. Biochem. Biotechnol., 165, 1141-1151.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 1.82
-
 methyl gallate at 45°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 2.06
-
 methyl gallate at 40°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 4.06
-
 methyl gallate at 50°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 4.35
-
 methyl gallate at 55°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 4.47
-
 methyl gallate at 60°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 4.94
-
 methyl gallate at 20°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 5.06
-
 methyl gallate at 35°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 5.11
-
 methyl gallate at 25°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 5.17
-
 methyl gallate at 30°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 12.05
-
 methyl gallate at 65°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + H2O Aspergillus niger
-
 gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O Aspergillus niger GH1
-
 gallate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus niger
-
-
-
3.1.1.20 Aspergillus niger GH1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 acetone precipitation and G25 Sephadex gel filtration Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 methyl gallate + H2O
-
 Aspergillus niger gallate + methanol
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Aspergillus niger GH1 gallate + methanol
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus niger gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus niger GH1 gallate + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 tannin acyl hydrolase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 60
-
 at a methyl gallate concentration of 4.4 mM Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 25 65
-
 Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.20 17.02
-
 methyl gallate at 20°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 52.48
-
 methyl gallate at 25°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 60.99
-
 methyl gallate at 35°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 78.01
-
 methyl gallate at 30°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 580.1
-
 methyl gallate at 40°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 601.4
-
 methyl gallate at 45°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 1109
-
 methyl gallate at 50°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 1364
-
 methyl gallate at 55°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 1564
-
 methyl gallate at 60°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 2611
-
 methyl gallate at 65°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 6
-
 at 30°C Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.20 2 8
-
 Aspergillus niger

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.20 3.45
-
 methyl gallate at 20°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 10.26
-
 methyl gallate at 25°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 12.06
-
 methyl gallate at 35°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 15.08
-
 methyl gallate at 30°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 216.7
-
 methyl gallate at 65°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 273.5
-
 methyl gallate at 50°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 282
-
 methyl gallate at 40°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 313.7
-
 methyl gallate at 55°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 330
-
 methyl gallate at 45°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
3.1.1.20 350.2
-
 methyl gallate at 60°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger