EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.3.1 | biotechnology | a simple and fast dynamically coated capillary electrophoretic method is developed for the characterization and inhibition studies of alkaline phosphatases | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.1 | arsenate | irreversible inhibitor | Bos taurus | |
3.1.3.1 | phosphate | competitive inhibitor | Bos taurus | |
3.1.3.1 | theophylline | reversible non-competitive inhibitor | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.1 | 0.082 | - |
4-nitrophenyl phosphate | pH 9.5, 37°C, Vmax: 0.96 nmol/min/mg enzyme, CIAP immobilized enzyme reactor (in excellent agreement with a Km value of calf ALP enzyme using a 96-well microplate reader) | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.1 | Bos taurus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.1 | intestine | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.1 | 4-nitrophenyl phosphate + H2O | - |
Bos taurus | 4-nitrophenol + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.1 | alkaline phosphatase | - |
Bos taurus |
3.1.3.1 | calf intestinal alkaline phosphatase | - |
Bos taurus |
3.1.3.1 | CIAP | - |
Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.1 | 37 | - |
assay at | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.1 | 9.5 | - |
assay at | Bos taurus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.1 | 0.000261 | - |
arsenate | pH 9.5, 37°C, standard spectrophotometric assays | Bos taurus | |
3.1.3.1 | 0.00241 | - |
phosphate | pH 9.5, 37°C, ALP immobilized capillary electrophoresis, Ki value is close to the Ki values obtained by the standard spectrophotometric assay | Bos taurus | |
3.1.3.1 | 0.00311 | - |
phosphate | pH 9.5, 37°C, standard spectrophotometric assays | Bos taurus | |
3.1.3.1 | 0.0089 | - |
arsenate | pH 9.5, 37°C, ALP immobilized capillary electrophoresis, Ki value is close to the Ki values obtained by the standard spectrophotometric assay | Bos taurus | |
3.1.3.1 | 0.05 | - |
theophylline | pH 9.5, 37°C, ALP immobilized capillary electrophoresis, Ki value is close to the Ki values obtained by the standard spectrophotometric assay | Bos taurus | |
3.1.3.1 | 0.096 | - |
theophylline | pH 9.5, 37°C, standard spectrophotometric assays | Bos taurus |