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Literature summary extracted from

  • Alias, N.; Mahadi, N.; Murad, A.; Bakar, F.; Rabu, A.; Illias, R.
    Expression optimisation of recombinant alpha-L-arabinofuranosidase from Aspergillus niger ATCC 120120 in Pichia pastoris and its biochemical characterization (2011), Afr. J. Biotechnol., 10, 6700-6710.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.55 Triton X-100 149.58% activity at 1 mM Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 expressed in Pichia pastoris X-33 cells Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Cu2+ 34.37% residual activity at 1 mM Aspergillus niger
3.2.1.55 DMSO 87.55% residual activity at 5% (v/v) Aspergillus niger
3.2.1.55 Fe2+ 34.37% residual activity at 1 mM Aspergillus niger
3.2.1.55 additional information the enzyme activity is relatively unaffected by EDTA Aspergillus niger
3.2.1.55 Urea 84.86% residual activity at 1 M Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.93
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM acetate buffer, pH 5.0, at 50°C Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.55 K+ 113.85% activity at 1% (v/v) Aspergillus niger
3.2.1.55 Mn2+ 136.48% activity at 1 mM Aspergillus niger
3.2.1.55 additional information the enzyme activity is relatively unaffected by Ca2+, CO2+, Mg2+ and EDTA Aspergillus niger
3.2.1.55 Na+ 111.17% activity at 1 mM Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 65334
-
 x * 65334, calculated from amino acid sequence Aspergillus niger
3.2.1.55 66000
-
 x * 66000, the purified recombinant abfA before and after treatment with PNGase F migrated by SDS-PAGE has relative molecular masses of about 83000 and 66000 Da, respectively Aspergillus niger
3.2.1.55 83000
-
 x * 83000, the purified recombinant abfA before and after treatment with PNGase F migrated by SDS-PAGE has relative molecular masses of about 83000 and 66000 Da, respectively Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aspergillus niger D6QST5
-
-
3.2.1.55 Aspergillus niger ATCC 120120 D6QST5
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.55 glycoprotein the enzyme is N-linked glycosylated Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger ATCC 120120 4-nitrophenol + alpha-L-arabinofuranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 ? x * 65334, calculated from amino acid sequence Aspergillus niger
3.2.1.55 ? x * 66000, the purified recombinant abfA before and after treatment with PNGase F migrated by SDS-PAGE has relative molecular masses of about 83000 and 66000 Da, respectively Aspergillus niger
3.2.1.55 ? x * 83000, the purified recombinant abfA before and after treatment with PNGase F migrated by SDS-PAGE has relative molecular masses of about 83000 and 66000 Da, respectively Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 AbfA
-
 Aspergillus niger
3.2.1.55 alpha-L-arabinofuranosidase
-
 Aspergillus niger
3.2.1.55 alpha-L-arabinofuranoside arabinofuranohydrolase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 50
-
 in 50 mM acetate buffer (pH 5.0) Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 40 50 the enzyme exhibits stability at temperatures up to 50°C, retaining 90% of its activity after pre-incubation at 40°C for 30 min. Higher temperatures result in a complete decrease in the enzyme activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 4
-
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.55 3 6 the enzyme is stable from pH 3.0 to 6.0 with a gradual loss of activity at higher and lower pH values. The enzyme is slowly inactivated at pH levels above 7.0 Aspergillus niger

Expression

EC Number Organism Comment Expression
3.2.1.55 Aspergillus niger gene abfA is expressed as an active enzyme at 28°C when cultured in BMMY medium (pH 6.0) and induced with 2% (v/v) methanol every 24 h. Maximum activity is observed 5 days after induction up