Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Saleem Basha, N.; Rekha, R.; Komala, M.; Ruby, S.
    Production of extracellular anti-leukaemic enzyme L-asparaginase from marine actinomycetes by solid-state and submerged fermentation: Purification and characterisation (2009), Trop. J. Pharm. Res., 8, 353-360.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.024
-
 L-asparagine pH 8.6, 37°C Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Cu2+ inhibits enzyme, 1, 3, and 10 mM tested Streptomyces sp.
3.5.1.1 EDTA inhibits enzyme, 1, 3, and 10 mM tested Streptomyces sp.
3.5.1.1 Mn2+ activates enzyme, 1, 3, and 10 mM tested Streptomyces sp.
3.5.1.1 Zn2+ inhibits enzyme, 1, 3, and 10 mM tested Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Streptomyces sp.
-
 marine actinomycetes, soil isolate S1, S2, S3, S4, S5, S6, S8. K2, K4, K5, and K8, whereof S3, S4 and S6 are designated as Streptomyces spp.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 to homogeneity by ammonium sulfate precipitation, dialysis, gel filtration on Sephadex G-100 and SDS-PAGE Streptomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.1 soil sediment samples obtained from Tamilnadu and Kerala in India, production of the enzyme in three different media, solid-State media, tryptone glucose yeast extract, and tryptone fructose yeast extract broth. 10 isolates (S1, S2, S3, S4, S5, S6, S8. K2, K4, K5, and K8), only S3, S4 and K8 show L-asparaginase activity Streptomyces sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 662.6
-
 purified enzyme, 37°C, pH 8.6 Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Streptomyces sp. L-aspartate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 L-asparaginase
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 37
-
 assay at Streptomyces sp.
3.5.1.1 50
-
-
 Streptomyces sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.1 30 75 ca. 30 U/ml at 30°C and 75°C Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 7.5
-
 maximum activity between pH 7.0 and 8.0 with 80% activity at physiological pH Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 5 10 20 U/ml enzyme activity at pH 5 and 10 Streptomyces sp.
3.5.1.1 8.6
-
 assay at Streptomyces sp.