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Literature summary extracted from

  • Dadashipour, M.; Fukuta, Y.; Asano, Y.
    Comparative expression of wild-type and highly soluble mutant His103Leu of hydroxynitrile lyase from Manihot esculenta in prokaryotic and eukaryotic expression systems (2010), Protein Expr. Purif., 77, 92-97.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.47 expressed in Pichia pastoris, Leishmania tarentolae, Escherichia coli strain JM109 and in two cell-free translations, including an Escherichia coli lysate (WakoPURE system) and wheat germ translation system Manihot esculenta
4.1.2.47 wild type and mutant H103L are expressed in Escherichia coli strain JM109, in Pichia pastoris and Leishmania tarentolae, as well as in an Escherichia coli lysate (WakoPURE system) and wheat germ translation system Manihot esculenta

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.47 H103L highly active and soluble mutant Manihot esculenta

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.47 Manihot esculenta
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.47 additional information
-
 no specific activity is detected with recombinant wild type and mutant enzyme H103L when expressed in an Escherichia coli lysate (WakoPURE system) or as N-terminal His6-tagged enzyme in a RTS 100 wheat germ cell-free translation system Manihot esculenta
4.1.2.47 0.7
-
 recombinant mutant enzyme H103L expressed in an Escherichia coli lysate (WakoPURE system), using benzaldehyde as substrate, pH and temperature not specified in the publication Manihot esculenta
4.1.2.47 1.02
-
 C-terminal His-tagged recombinant mutant enzyme H103L expressed in a RTS 100 wheat germ cell-free translation system, using benzaldehyde as substrate, pH and temperature not specified in the publication Manihot esculenta
4.1.2.47 1.1
-
 recombinant mutant enzyme H103L expressed in Pichia pastoris, using benzaldehyde as substrate, pH and temperature not specified in the publication Manihot esculenta
4.1.2.47 1.55
-
 C-terminal His-tagged recombinant wild type enzyme expressed in a RTS 100 wheat germ cell-free translation system, using benzaldehyde as substrate, pH and temperature not specified in the publication Manihot esculenta
4.1.2.47 1.73
-
 recombinant wild type enzyme expressed in Escherichia coli JM109 cells, using benzaldehyde as substrate, pH and temperature not specified in the publication Manihot esculenta
4.1.2.47 1.97
-
 untagged recombinant wild type enzyme expressed in a RTS 100 wheat germ cell-free translation system, using benzaldehyde as substrate, pH and temperature not specified in the publication Manihot esculenta
4.1.2.47 1.98
-
 untagged recombinant mutant enzyme H103L expressed in a RTS 100 wheat germ cell-free translation system, using benzaldehyde as substrate, pH and temperature not specified in the publication Manihot esculenta
4.1.2.47 2.03
-
 recombinant wild type enzyme expressed in Leishmania tarentolae, using benzaldehyde as substrate, pH and temperature not specified in the publication Manihot esculenta
4.1.2.47 2.1
-
 recombinant wild type enzyme expressed in Pichia pastoris, using benzaldehyde as substrate, pH and temperature not specified in the publication Manihot esculenta
4.1.2.47 2.18
-
 recombinant mutant enzyme H103L expressed in Leishmania tarentolae, using benzaldehyde as substrate, pH and temperature not specified in the publication Manihot esculenta
4.1.2.47 29.2
-
 recombinant mutant enzyme H103L expressed in Escherichia coli JM109 cells, using benzaldehyde as substrate, pH and temperature not specified in the publication Manihot esculenta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.47 (S)-mandelonitrile
-
 Manihot esculenta cyanide + benzaldehyde
-
 r
4.1.2.47 (S)-mandelonitrile
-
 Manihot esculenta benzaldehyde + HCN
-
 ?
4.1.2.47 cyanide + benzaldehyde
-
 Manihot esculenta (S)-mandelonitrile
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.1.2.47 HNL
-
 Manihot esculenta
4.1.2.47 Hydroxynitrile lyase
-
 Manihot esculenta
4.1.2.47 S-HnL
-
 Manihot esculenta