EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.15.1 | expression of wild-type enzyme, encoded by gene camC, with gene camB, encoding the putidaredoxin, in Escherichia coli strains BL21(DE3), expression of mutant enzymes in Escherichia coli strain DH10B | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.15.1 | I396A | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
1.14.15.1 | I396G | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
1.14.15.1 | I396V | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
1.14.15.1 | M395I | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
1.14.15.1 | M96Y | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
1.14.15.1 | additional information | establishment of an in vitro screening system for P450cam selecting variants by the activity on NADH | Pseudomonas putida |
1.14.15.1 | N244L | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
1.14.15.1 | T297D | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
1.14.15.1 | V87F | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.1 | (1R)-camphor + putidaredoxin + O2 | Pseudomonas putida | putidaredoxin transfers electrons from NADH to P450cam in a coupled assay method | 5-exo-(1R)-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
1.14.15.1 | (1R)-camphor + putidaredoxin + O2 | Pseudomonas putida PpG1 | putidaredoxin transfers electrons from NADH to P450cam in a coupled assay method | 5-exo-(1R)-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.1 | Pseudomonas putida | - |
gene camC | - |
1.14.15.1 | Pseudomonas putida PpG1 | - |
gene camC | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.15.1 | additional information | cells grown in D-camphor minimal medium | Pseudomonas putida | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.1 | (1R)-camphor + putidaredoxin + O2 | putidaredoxin transfers electrons from NADH to P450cam in a coupled assay method | Pseudomonas putida | 5-exo-(1R)-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
1.14.15.1 | (1R)-camphor + putidaredoxin + O2 | putidaredoxin transfers electrons from NADH to P450cam | Pseudomonas putida | 5-exo-(1R)-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
1.14.15.1 | (1R)-camphor + putidaredoxin + O2 | putidaredoxin transfers electrons from NADH to P450cam in a coupled assay method | Pseudomonas putida PpG1 | 5-exo-(1R)-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
1.14.15.1 | (1R)-camphor + putidaredoxin + O2 | putidaredoxin transfers electrons from NADH to P450cam | Pseudomonas putida PpG1 | 5-exo-(1R)-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
1.14.15.1 | additional information | substrate specificities of wild-type and mutant enzymes, overview | Pseudomonas putida | ? | - |
? | |
1.14.15.1 | additional information | substrate specificities of wild-type and mutant enzymes, overview | Pseudomonas putida PpG1 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.15.1 | CYP101 | - |
Pseudomonas putida |
1.14.15.1 | P450cam | - |
Pseudomonas putida |
1.14.15.1 | P450cam monooxygenase | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | 30 | - |
assay at | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | 7.4 | - |
assay at | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.1 | putidaredoxin | transferring electrons from NADH to P450cam in a coupled assay method | Pseudomonas putida |