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Literature summary extracted from

  • Hsieh, J.M.; Tsirulnikov, K.; Sawaya, M.R.; Magilnick, N.; Abuladze, N.; Kurtz, I.; Abramson, J.; Pushkin, A.
    Structures of aminoacylase 3 in complex with acetylated substrates (2010), Proc. Natl. Acad. Sci. USA, 107, 17962-17967.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.114 expression in Escherichia coli Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.14 hanging drop vapor diffusion technique Mus musculus
3.5.1.114 crystal structure of recombinant mouse AA3 (mAA3) in the presence of its acetate byproduct and two substrates: Nalpha-acetyl-L-tyrosine and N-acetyl-S-1,2-dichlorovinyl-L-cysteine Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.14 E167R involved in substrate specificity Mus musculus
3.5.1.14 E177A catalytically inactive but maintained the structural integrity of active site Mus musculus
3.5.1.14 E177D reduces the kcat value more than threefold Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.14 L-benzylsuccinate
-
 Mus musculus
3.5.1.114 additional information trichloroethylene is one of the most widespread environmental contaminants, which is metabolized to N-acetyl-S-1,2-dichlorovinyl-L-cysteine (NA-DCVC) before being excreted in the urine. Alternatively, NA-DCVC can be deacetylated by aminoacylase 3, an enzyme that is highly expressed in the kidney, liver, and brain. NA-DCVC deacetylation initiates the transformation into toxic products that ultimately causes acute renal failure. Aminoacylase 3 inhibition is therefore a target of interest to prevent TCE induced nephrotoxicity Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Mus musculus
-
-
-
3.5.1.114 Mus musculus Q91XE4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.14 StrepTactin Sepharose Mus musculus
3.5.1.114
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.114 brain
-
 Mus musculus
-
3.5.1.114 kidney
-
 Mus musculus
-
3.5.1.114 liver
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 N-acetyl-L-aspartate + H2O no activity of wild type protein, mutant E167R protein shows activity Mus musculus acetate + L-aspartate
-
 ?
3.5.1.14 N-acetyl-L-tyrosine + H2O
-
 Mus musculus acetate + L-tyrosine
-
 ?
3.5.1.14 N-acetyl-S-1,2-dichlorovinyl-L-cysteine + H2O
-
 Mus musculus acetate + S-1,2-dichlorovinyl-L-cysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.14 aminoacylase 3
-
 Mus musculus
3.5.1.114 AA3
-
 Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.14 0.1
-
 N-acetyl-L-aspartate E167R mutant protein, pH not specified in the publication, temperature not specified in the publication Mus musculus
3.5.1.14 0.6
-
 N-acetyl-L-tyrosine E167R mutant protein, pH not specified in the publication, temperature not specified in the publication Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.14 0.025
-
 L-benzylsuccinate pH not specified in the publication, temperature not specified in the publication Mus musculus