EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.98.1 | expression of HdrB1 and HdrB2 as C-terminally His6-tagged proteins in strain MM1263 and MM1264, respectively. The His tag does not affect enzyme activity or binding | Methanococcus maripaludis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.98.1 | additional information | construction of two mutant strains: MM1263 containing His-tagged HdrB1, MM1264 containing His-tagged HdrB2. The strain containing His-tagged HdrB1 and a null mutation in hdrB2 grows normally, as does the strain containing His-tagged HdrB2 and a null mutation in hdrB1. Because Hdr is essential, each His-tagged protein must be functional | Methanococcus maripaludis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.12 | Tungsten | Fwd is a tungsten-containing enzyme | Methanothermobacter marburgensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.12 | CO2 + methanofuran + reduced ferredoxin | Methanothermobacter marburgensis | - |
formylmethanofuran + H2O + oxidized ferredoxin | - |
? | |
1.2.7.12 | CO2 + methanofuran + reduced ferredoxin | Methanothermobacter marburgensis S2 | - |
formylmethanofuran + H2O + oxidized ferredoxin | - |
? | |
1.8.98.1 | coenzyme B + coenzyme M + methanophenazine | Methanococcus maripaludis | - |
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine | - |
? | |
1.8.98.1 | coenzyme B + coenzyme M + methanophenazine | Methanococcus maripaludis MM1265 | - |
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine | - |
? | |
1.8.98.1 | additional information | Methanococcus maripaludis | Hdr complexes with F420-nonreducing hydrogenase, formate dehydrogenase, and formyl-MFR dehydrogenase, model of complex of proteins that interact with Hdr, overview | ? | - |
? | |
1.8.98.1 | additional information | Methanococcus maripaludis MM1265 | Hdr complexes with F420-nonreducing hydrogenase, formate dehydrogenase, and formyl-MFR dehydrogenase, model of complex of proteins that interact with Hdr, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.12 | Methanothermobacter marburgensis | - |
- |
- |
1.2.7.12 | Methanothermobacter marburgensis S2 | - |
- |
- |
1.8.98.1 | Methanococcus maripaludis | - |
- |
- |
1.8.98.1 | Methanococcus maripaludis MM1265 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.12 | Poly-Prep resin column chromatography | Methanothermobacter marburgensis |
1.8.98.1 | recombinant C-terminally His6-tagged HdrB1 and HdrB2 by nickel affinity chromatography | Methanococcus maripaludis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.12 | CO2 + methanofuran + reduced ferredoxin | - |
Methanothermobacter marburgensis | formylmethanofuran + H2O + oxidized ferredoxin | - |
? | |
1.2.7.12 | CO2 + methanofuran + reduced ferredoxin | - |
Methanothermobacter marburgensis S2 | formylmethanofuran + H2O + oxidized ferredoxin | - |
? | |
1.8.98.1 | coenzyme B + coenzyme M + methanophenazine | - |
Methanococcus maripaludis | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine | - |
? | |
1.8.98.1 | coenzyme B + coenzyme M + methanophenazine | - |
Methanococcus maripaludis MM1265 | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine | - |
? | |
1.8.98.1 | additional information | Hdr complexes with F420-nonreducing hydrogenase, formate dehydrogenase, and formyl-MFR dehydrogenase, model of complex of proteins that interact with Hdr, overview | Methanococcus maripaludis | ? | - |
? | |
1.8.98.1 | additional information | Hdr complexes with F420-nonreducing hydrogenase, formate dehydrogenase, and formyl-MFR dehydrogenase, model of complex of proteins that interact with Hdr, overview | Methanococcus maripaludis MM1265 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.7.12 | formyl-MFR dehydrogenase | - |
Methanothermobacter marburgensis |
1.2.7.12 | Fwd | - |
Methanothermobacter marburgensis |
1.8.98.1 | HDR | - |
Methanococcus maripaludis |
1.8.98.1 | heterodisulfide reductase | - |
Methanococcus maripaludis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.8.98.1 | metabolism | in methanogenic Archaea, the final step of methanogenesis generates methane and a heterodisulfide of coenzyme M and coenzyme B, CoM-S-S-CoB. Reduction of this heterodisulfide by heterodisulfide reductase regenerates HS-CoM and HS-CoB in an exergonic process, methanogenic pathway with hypothesized roles for the heterodisulfide reductase complex, overview. Hdr complexes with F420-nonreducing hydrogenase, formate dehydrogenase, and formyl-methanofuran dehydrogenase, model of complex of proteins that interact with Hdr, overview | Methanococcus maripaludis |
1.8.98.1 | additional information | a strain containing His-tagged HdrB1 and a null mutation in hdrB2 grows normally, as does a strain containing His-tagged HdrB2 and a null mutation in hdrB1. Because Hdr is essential, each His-tagged protein must be functional | Methanococcus maripaludis |