Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mayoral, J.G.; Nouzova, M.; Navare, A.; Noriega, F.G.
    NADP+-dependent farnesol dehydrogenase, a corpora allata enzyme involved in juvenile hormone synthesis (2009), Proc. Natl. Acad. Sci. USA, 106, 21091-21096.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.216 expression in Escherichia coli Aedes aegypti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.216 0.091
-
 (2E,6E)-farnesol pH 10.0, 30°C Aedes aegypti
1.1.1.216 0.097
-
 (2Z,6Z)-farnesol pH 10.0, 30°C Aedes aegypti
1.1.1.216 0.109
-
 (Z)-nerol pH 10.0, 30°C Aedes aegypti
1.1.1.216 0.133
-
 citronellol pH 10.0, 30°C Aedes aegypti
1.1.1.216 0.184
-
 2-decanol pH 10.0, 30°C Aedes aegypti
1.1.1.216 0.195
-
 Octanol pH 10.0, 30°C Aedes aegypti
1.1.1.216 0.208
-
 (E)-geraniol pH 10.0, 30°C Aedes aegypti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.216 29000
-
 2 * 29000, calculated Aedes aegypti
1.1.1.216 60000
-
 gel filtration Aedes aegypti

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.216 Aedes aegypti D2WKD9 NADP+-dependent farnesol dehydrogenase 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.216 recombinant enzyme Aedes aegypti

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.216 brain and midgut, of adult female, highest levels of mRNA Aedes aegypti
-
1.1.1.216 corpora allata no farnesol dehydrogenase activity in newly emerged mosquitoes but significant activity 24 h later. SDR-1 mRNA levels are very low in the inactive corpora allata of the newly emerged female, but increased over 30-fold 24 h later during the peak of juvenile hormone synthesis Aedes aegypti
-
1.1.1.216 fat body and Malpighian tubules, considerable levles of mRNA Aedes aegypti
-
1.1.1.216 ganglion abdominal and thoracic ganglia, high level of mRNA Aedes aegypti
-
1.1.1.216 malpighian tubule and fat body, considerable levles of mRNA Aedes aegypti
-
1.1.1.216 midgut and brain, of adult female, highest levels of mRNA Aedes aegypti
-
1.1.1.216 testis of adult male, substantial level of mRNA Aedes aegypti
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.216 9
-
 substrate (2Z,6Z)-farnesol, pH 10.0, 30°C Aedes aegypti
1.1.1.216 9.7
-
 substrate (2E,6E)-farnesol, pH 10.0, 30°C Aedes aegypti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.216 (2E,6E)-farnesol + NADP+ enzyme shows highest affinity for farnesol Aedes aegypti (2E,6E)-farnesal + NADPH + H+
-
 ir
1.1.1.216 (2Z,6Z)-farnesol + NADP+ enzyme shows highest affinity for farnesol Aedes aegypti (2Z,6Z)-farnesal + NADPH + H+
-
 r
1.1.1.216 (E)-geraniol + NADP+
-
 Aedes aegypti (E)-geranial + NADPH + H+
-
 ?
1.1.1.216 (Z)-nerol + NADP+
-
 Aedes aegypti (Z)-neral + NADPH + H+
-
 ?
1.1.1.216 2-decanol + NADP+
-
 Aedes aegypti ? + NADPH + H+
-
 ?
1.1.1.216 citronellol + NADP+
-
 Aedes aegypti citronellal + NADPH + H+
-
 ?
1.1.1.216 additional information no activity is detected for butanol, ethanol, or glycerol, or for farnesal or farnesoic acid Aedes aegypti ?
-
 ?
1.1.1.216 octanol + NADP+
-
 Aedes aegypti octanal + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.216 dimer 2 * 29000, calculated Aedes aegypti

Synonyms

EC Number Synonyms Comment Organism
1.1.1.216 NADP+-dependent farnesol dehydrogenase 1
-
 Aedes aegypti
1.1.1.216 SDR-1
-
 Aedes aegypti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.216 10 11
-
 Aedes aegypti

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.216 NADP+ absolutely required, NAD+ or FAD cannot substitute Aedes aegypti

Expression

EC Number Organism Comment Expression
1.1.1.216 Aedes aegypti SDR-1 mRNA levels are very low in the inactive corpora allata of the newly emerged female, but increased over 30-fold 24 h later during the peak of juvenile hormone synthesis additional information