Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Diwakar, S.K.; Mishra, S.K.
    Purification and biochemical characterization of ionically unbound polyphenol oxidase from Musa paradisiaca leaf (2011), Prep. Biochem. Biotechnol., 41, 187-200.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.1 ferulic acid activates Musa x paradisiaca
1.10.3.1 protocatechuic acid activates Musa x paradisiaca
1.14.18.1 ferrulic acid enhances enzyme activity Musa x paradisiaca
1.14.18.1 protocatechuate enhances enzyme activity Musa x paradisiaca

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 2-mercaptoethanol
-
 Musa x paradisiaca
1.10.3.1 ascorbic acid
-
 Musa x paradisiaca
1.10.3.1 Co2+
-
 Musa x paradisiaca
1.10.3.1 EDTA
-
 Musa x paradisiaca
1.10.3.1 K+
-
 Musa x paradisiaca
1.10.3.1 kojic acid
-
 Musa x paradisiaca
1.10.3.1 L-cysteine
-
 Musa x paradisiaca
1.10.3.1 Na+
-
 Musa x paradisiaca
1.10.3.1 Sodium azide
-
 Musa x paradisiaca
1.14.18.1 2-mercaptoethanol
-
 Musa x paradisiaca
1.14.18.1 5-hydroxy-2-(hydroxymethyl)-4H-pyran-4-one
-
 Musa x paradisiaca
1.14.18.1 ascorbic acid
-
 Musa x paradisiaca
1.14.18.1 Co2+
-
 Musa x paradisiaca
1.14.18.1 EDTA
-
 Musa x paradisiaca
1.14.18.1 K+
-
 Musa x paradisiaca
1.14.18.1 L-cysteine
-
 Musa x paradisiaca
1.14.18.1 Na+
-
 Musa x paradisiaca
1.14.18.1 Sodium azide
-
 Musa x paradisiaca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 8
-
 catechol pH 7.0, 50°C Musa x paradisiaca
1.10.3.1 10
-
 4-methylcatechol pH 7.0, 50°C Musa x paradisiaca
1.14.18.1 8
-
 catechol pH 7.0, 50°C Musa x paradisiaca
1.14.18.1 10
-
 4-methylcatechol pH 7.0, 50°C Musa x paradisiaca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.1 Cu2+ activates Musa x paradisiaca
1.10.3.1 Fe2+ activates Musa x paradisiaca
1.10.3.1 Hg2+ activates Musa x paradisiaca
1.10.3.1 Mn2+ activates Musa x paradisiaca
1.10.3.1 Ni2+ activates Musa x paradisiaca
1.14.18.1 Cu2+ enhances enzyme activity Musa x paradisiaca
1.14.18.1 Fe2+ enhances enzyme activity Musa x paradisiaca
1.14.18.1 Hg2+ enhances enzyme activity Musa x paradisiaca
1.14.18.1 Mn2+ enhances enzyme activity Musa x paradisiaca
1.14.18.1 Ni2+ enhances enzyme activity Musa x paradisiaca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 40000
-
 ? * 40000, SDS-PAGE Musa x paradisiaca
1.14.18.1 40000
-
 gel filtration Musa x paradisiaca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.1 2 4-methylcatechol + O2 Musa x paradisiaca
-
 2 4-methyl-1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.1 2 catechol + O2 Musa x paradisiaca
-
 2 1,2-benzoquinone + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Musa x paradisiaca
-
-
-
1.14.18.1 Musa x paradisiaca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 native enzyme from leaves 2.54fold by ammonium sulfate fractionation and gel filtration Musa x paradisiaca
1.14.18.1 ammonium sulfate precipitation and Sephadex G-100 gel filtration Musa x paradisiaca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 leaf
-
 Musa x paradisiaca
-
1.14.18.1 leaf
-
 Musa x paradisiaca
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 2 4-methylcatechol + O2
-
 Musa x paradisiaca 2 4-methyl-1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.1 2 catechol + O2
-
 Musa x paradisiaca 2 1,2-benzoquinone + 2 H2O
-
 ?
1.14.18.1 2 catechol + O2
-
 Musa x paradisiaca 2 1,2-benzoquinone + 2 H2O
-
 ?
1.14.18.1 4-methylcatechol + O2
-
 Musa x paradisiaca 4-methyl-1,2-benzoquinone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.1 ? ? * 40000, SDS-PAGE Musa x paradisiaca
1.14.18.1 monomer 1 * 40000, SDS-PAGE Musa x paradisiaca

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 polyphenol oxidase
-
 Musa x paradisiaca
1.14.18.1 PPO
-
 Musa x paradisiaca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 50
-
-
 Musa x paradisiaca
1.14.18.1 50
-
-
 Musa x paradisiaca

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.1 30 90 activity range Musa x paradisiaca
1.14.18.1 30 90
-
 Musa x paradisiaca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.1 60 75 half-life values of the enzyme range from 17 to 77 min Musa x paradisiaca
1.14.18.1 60 75 in the range from 60 to 75°C, the half-life of the enzyme reaches from 17 to 77 min Musa x paradisiaca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 7
-
-
 Musa x paradisiaca
1.14.18.1 7
-
-
 Musa x paradisiaca

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.1 4 9 activity range Musa x paradisiaca
1.14.18.1 4 9
-
 Musa x paradisiaca