EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.20.6 | gene ZmFLS1, ZmFLS1 is under the control of the anthocyanin (C1/PL1 + R/B) and 3-deoxy flavonoid (P1) transcriptional regulators in vivo, recombinant expression as His-tagged protein in Escherichia coli | Zea mays |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.20.6 | H132F | site-directed mutagenesis, the mutant shows similar kinetics with dihydrokaempferol compared to the wild-type enzyme | Zea mays |
1.14.20.6 | additional information | ZmFLS1 partially complements the flavonol deficiency of the Arabidopsis thaliana fls1 mutant, and restores anthocyanin accumulation to normal levels. ZmFLS1 is an immediate direct target of the P1 and C1/R regulatory complexes | Zea mays |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.20.6 | 0.0584 | - |
dihydrokaempferol | recombinant FLS1, pH not specified in the publication, temperature not specified in the publication | Zea mays | |
1.14.20.6 | 0.1511 | - |
dihydroquercetin | recombinant FLS1, pH not specified in the publication, temperature not specified in the publication | Zea mays |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.20.6 | additional information | Zea mays | FLS1 is capable of converting the dihydrokaempferol and dihydroquercetin dihydroflavonols to the corresponding flavonols, kaempferol and quercetin | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.20.6 | Zea mays | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.20.6 | recombinant His-tagged FLS1 from Escherichia coli to about 80% purity by nickel affinity chromatography | Zea mays |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.20.6 | dihydrokaempferol + 2-oxoglutarate + O2 | - |
Zea mays | kaempferol + succinate + CO2 + H2O | - |
? | |
1.14.20.6 | dihydroquercetin + 2-oxoglutarate + O2 | - |
Zea mays | quercetin + succinate + CO2 + H2O | - |
? | |
1.14.20.6 | additional information | FLS1 is capable of converting the dihydrokaempferol and dihydroquercetin dihydroflavonols to the corresponding flavonols, kaempferol and quercetin | Zea mays | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.20.6 | flavonol synthase | - |
Zea mays |
1.14.20.6 | FLS1 | - |
Zea mays |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.20.6 | 3.9 | - |
dihydroquercetin | recombinant FLS1, pH not specified in the publication, temperature not specified in the publication | Zea mays | |
1.14.20.6 | 6.6 | - |
dihydrokaempferol | recombinant FLS1, pH not specified in the publication, temperature not specified in the publication | Zea mays |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.20.6 | Zea mays | the enzyme is UV-B-light-inducible in maize seedlings | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.20.6 | metabolism | ZmFLS1 is a key flavonoid biosynthetic enzyme and involved in control of flavonol accumulation in maize | Zea mays |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.20.6 | 26 | - |
dihydroquercetin | recombinant FLS1, pH not specified in the publication, temperature not specified in the publication | Zea mays | |
1.14.20.6 | 113 | - |
dihydrokaempferol | recombinant FLS1, pH not specified in the publication, temperature not specified in the publication | Zea mays |