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Literature summary extracted from
- Identification of the chlB gene and the gene product essential for the light-independent chlorophyll biosynthesis in the cyanobacterium Plectonema boryanum (1996), Plant Cell Physiol., 37, 313-323.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.3.7.7 | His-tagged subunit ChlB fusion protein is expressed in Escherichia coli JM105 cells | Leptolyngbya boryana |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.3.7.7 | membrane | cytoplasmic or thylakoid membranes | Leptolyngbya boryana | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.7.7 | 56820 | - |
subunit ChlB, calculated from amino acid sequence | Leptolyngbya boryana |
| 1.3.7.7 | 58000 | - |
His-tagged subunit ChlB fusion protein, SDS-PAGE | Leptolyngbya boryana |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.7.7 | protochlorophyllide + oxidized ferredoxin + ADP + phosphate | Leptolyngbya boryana | - |
chlorophyllide a + reduced ferredoxin + ATP | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.7.7 | Leptolyngbya boryana | - |
strain IAM-M101 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.7.7 | HiTrap chelating column chromatography and Superdex 200pg gel filtration | Leptolyngbya boryana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.7.7 | protochlorophyllide + oxidized ferredoxin + ADP + phosphate | - |
Leptolyngbya boryana | chlorophyllide a + reduced ferredoxin + ATP | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.3.7.7 | light-independent Pchlide reductase | - |
Leptolyngbya boryana |
| 1.3.7.7 | light-independent protochlorophyllide reductase | - |
Leptolyngbya boryana |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.7.7 | ADP | - |
Leptolyngbya boryana | |
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Release 2023.1 (January 2023)
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