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Literature summary extracted from

  • Zhang, D.; Grode, K.D.; Stewman, S.F.; Diaz-Valencia, J.D.; Liebling, E.; Rath, U.; Riera, T.; Currie, J.D.; Buster, D.W.; Asenjo, A.B.; Sosa, H.J.; Ross, J.L.; Ma, A.; Rogers, S.L.; Sharp, D.J.
    Drosophila katanin is a microtubule depolymerase that regulates cortical-microtubule plus-end interactions and cell migration (2011), Nat. Cell Biol., 13, 361-369.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.1 baculovirus-expressed Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.1.1 cell cortex Dm-Kat60 concentrates at the cell cortex of S2 Drosophila cells during interphase, where it suppresses the polymerization of microtubule plus-ends Drosophila melanogaster 5938
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5.6.1.1 additional information Dm-Kat60 also generally co-localizes with cortical actin. Cortical localization od Dm-Kat60 occurs indepently of microtubules, as it persists following microtubule depolymerization with colchicine Drosophila melanogaster
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5.6.1.1 additional information Dm-Kat60 also localizes at the leading edge of migratory D17 Drosophila cells and negatively regulates multiple parameters of their motility Drosophila melanogaster
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Organism

EC Number Organism UniProt Comment Textmining
5.6.1.1 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.1 baculovirus-expressed, recombinant DM-Kat60 Drosophila melanogaster

Reaction

EC Number Reaction Comment Organism Reaction ID
5.6.1.1 n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers Dm-Kat60 severs and depolymerizes microtubules from their ends Drosophila melanogaster
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.1.1 Drosophila D17 cell
-
 Drosophila melanogaster
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5.6.1.1 SCHNEIDER-2 cell S2 cell Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.1 ATP + H2O recombinant Dm-Kat60 robustly severs taxol-stabilized microtubules in the presence of ATP. This reacton is inhibited when ATP is depleted with hexokinase and glucose or replaced with the non-hydrolysable ATP analogue 5'-adenylyl-beta-gamma-imidotriphosphate AMPPMP Drosophila melanogaster ADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.1 Dm-Kat60
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 Drosophila melanogaster
5.6.1.1 Drosophila katanin Dm-Kat60
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 Drosophila melanogaster
5.6.1.1 katanin
-
 Drosophila melanogaster