EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.B75 | gene POMT7, error-prone polymerase chain reaction, expression of GST-tagged wild-type POMT7 and mutant POMT7-M1 in Escherichia coli | Populus sp. |
2.1.1.B76 | - |
Populus sp. |
2.1.1.B76 | gene POMT7, error-prone polymerase chain reaction, expression of GST-tagged wild-type POMT7 and mutant POMT7-M1 in Escherichia coli | Populus sp. |
2.1.1.82 | expressed in Escherichia coli BL21 cells | Populus sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.B75 | D257G | random mutagenesis, the mutant of POMT-7, POMT7-M1, has a novel regioselectivity, from large scale mutant screening, mutant D257G methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group | Populus sp. |
2.1.1.B76 | D257G | random mutagenesis, the mutant of POMT-7, POMT7-M1, has a novel regioselectivity, from large scale mutant screening, mutant D257G methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group | Populus sp. |
2.1.1.B76 | N257G | the mutant methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group. The wild type POMT7 exhibits better activity toward kaempferol and quercetin than the mutant enzyme | Populus sp. |
2.1.1.82 | D257G | the mutant methylates the 3-hydroxyl group of flavonols in addition to the 7-hydrdoxyl group | Populus sp. |
2.1.1.82 | L101M | the mutant shows wild type reactivity with kaempferol and quercetin | Populus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.B75 | 0.0298 | - |
kaempferol | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
2.1.1.B75 | 0.0324 | - |
kaempferol | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
2.1.1.B75 | 0.0324 | - |
quercetin | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
2.1.1.B75 | 0.0378 | - |
quercetin | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
2.1.1.B76 | 0.0298 | - |
2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-1-benzopyran-4-one | i.e. quercetin, pH 7.5, 37°C, wild-type enzyme | Populus sp. | |
2.1.1.B76 | 0.0298 | - |
kaempferol | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
2.1.1.B76 | 0.0324 | - |
3,4',5,7-tetrahydroxyflavanone | i.e. kaempferol, pH 7.5, 37°C, wild-type enzyme | Populus sp. | |
2.1.1.B76 | 0.0324 | - |
2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-1-benzopyran-4-one | i.e. quercetin, pH 7.5, 37°C, mutant enzyme N257G | Populus sp. | |
2.1.1.B76 | 0.0324 | - |
kaempferol | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
2.1.1.B76 | 0.0324 | - |
quercetin | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
2.1.1.B76 | 0.0378 | - |
3,4',5,7-tetrahydroxyflavanone | i.e. kaempferol, pH 7.5, 37°C, mutant enzyme N257G | Populus sp. | |
2.1.1.B76 | 0.0378 | - |
quercetin | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
2.1.1.82 | 0.0298 | - |
quercetin | wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
2.1.1.82 | 0.0324 | - |
quercetin | mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
2.1.1.82 | 0.0324 | - |
kaempferol | wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
2.1.1.82 | 0.0378 | - |
kaempferol | mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.82 | 60000 | - |
x * 60000, SDS-PAGE | Populus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.B75 | S-adenosyl-L-methionine + kaempferol | Populus sp. | - |
S-adenosyl-L-homocysteine + 7-O-methylkaempferol | - |
? | |
2.1.1.B75 | S-adenosyl-L-methionine + quercetin | Populus sp. | - |
S-adenosyl-L-homocysteine + 7-O-methylquercetin | - |
? | |
2.1.1.B76 | S-adenosyl-L-methionine + kaempferol | Populus sp. | - |
S-adenosyl-L-homocysteine + 7-O-methylkaempferol | - |
? | |
2.1.1.B76 | S-adenosyl-L-methionine + quercetin | Populus sp. | - |
S-adenosyl-L-homocysteine + 7-O-methylquercetin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.B75 | Populus sp. | - |
- |
- |
2.1.1.B76 | Populus sp. | - |
- |
- |
2.1.1.82 | Populus sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.B75 | recombinant GST-tagged wild-type POMT7 and mutant POMT7-M1 fromEscherichia coli by glutathione affinity chromatography | Populus sp. |
2.1.1.B76 | recombinant GST-tagged wild-type POMT7 and mutant POMT7-M1 from Escherichia coli by glutathione affinity chromatography | Populus sp. |
2.1.1.B76 | recombinent POMT7 and mutants | Populus sp. |
2.1.1.82 | GST affinity column chromatography | Populus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.B75 | 2 S-adenosyl-L-methionine + kaempferol | activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7 | Populus sp. | 2 S-adenosyl-L-homocysteine + 3,7-O-dimethylkaempferol | - |
? | |
2.1.1.B75 | 2 S-adenosyl-L-methionine + quercetin | activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7, molecular docking of quercetin into the modeled structure of Asp257Gly | Populus sp. | 2 S-adenosyl-L-homocysteine + 3,7-O-dimethylquercetin | - |
? | |
2.1.1.B75 | S-adenosyl-L-methionine + kaempferol | - |
Populus sp. | S-adenosyl-L-homocysteine + 7-O-methylkaempferol | - |
? | |
2.1.1.B75 | S-adenosyl-L-methionine + quercetin | - |
Populus sp. | S-adenosyl-L-homocysteine + 7-O-methylquercetin | - |
? | |
2.1.1.B76 | 2 S-adenosyl-L-methionine + kaempferol | activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7 | Populus sp. | S-adenosyl-L-homocysteine + 3,7-O-dimethylkaempferol | - |
? | |
2.1.1.B76 | 2 S-adenosyl-L-methionine + quercetin | activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7, molecular docking of quercetin into the modeled structure of Asp257Gly | Populus sp. | S-adenosyl-L-homocysteine + 3,7-O-dimethylquercetin | - |
? | |
2.1.1.B76 | S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone | i.e. quercetin | Populus sp. | S-adenosyl-L-homocysteine + 3,3',4',5-tetrahydroxy-7-methoxyflavone | - |
? | |
2.1.1.B76 | S-adenosyl-L-methionine + 3,4',5,7-tetrahydroxyflavanone | i.e. kaempferol | Populus sp. | S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone | - |
? | |
2.1.1.B76 | S-adenosyl-L-methionine + kaempferol | - |
Populus sp. | S-adenosyl-L-homocysteine + 7-O-methylkaempferol | - |
? | |
2.1.1.B76 | S-adenosyl-L-methionine + quercetin | - |
Populus sp. | S-adenosyl-L-homocysteine + 7-O-methylquercetin | - |
? | |
2.1.1.82 | S-adenosyl-L-methionine + kaempferol | - |
Populus sp. | 7-O-methylkaempferol | - |
? | |
2.1.1.82 | S-adenosyl-L-methionine + quercetin | - |
Populus sp. | 7-O-methylquercetin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.82 | ? | x * 60000, SDS-PAGE | Populus sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.B75 | flavonoid O-methyltransferase | - |
Populus sp. |
2.1.1.B75 | POMT7 | - |
Populus sp. |
2.1.1.B76 | flavonoid O-methyltransferase | - |
Populus sp. |
2.1.1.B76 | POMT7 | - |
Populus sp. |
2.1.1.82 | flavone 7-O-methyltransferase | - |
Populus sp. |
2.1.1.82 | MT7 | - |
Populus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.B75 | 37 | - |
assay at | Populus sp. |
2.1.1.B76 | 37 | - |
assay at | Populus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.B75 | 0.064 | - |
kaempferol | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
2.1.1.B75 | 0.064 | - |
quercetin | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
2.1.1.B75 | 0.07 | - |
quercetin | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
2.1.1.B75 | 0.071 | - |
kaempferol | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
2.1.1.B76 | 0.064 | - |
3,4',5,7-tetrahydroxyflavanone | i.e. kaempferol, pH 7.5, 37°C, wild-type enzyme | Populus sp. | |
2.1.1.B76 | 0.064 | - |
2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-1-benzopyran-4-one | i.e. quercetin, pH 7.5, 37°C, mutant enzyme N257G | Populus sp. | |
2.1.1.B76 | 0.064 | - |
kaempferol | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
2.1.1.B76 | 0.064 | - |
quercetin | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
2.1.1.B76 | 0.07 | - |
3,4',5,7-tetrahydroxyflavanone | i.e. kaempferol, pH 7.5, 37°C, mutant enzyme N257G | Populus sp. | |
2.1.1.B76 | 0.07 | - |
quercetin | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
2.1.1.B76 | 0.071 | - |
2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-1-benzopyran-4-one | i.e. quercetin, pH 7.5, 37°C, wild-type enzyme | Populus sp. | |
2.1.1.B76 | 0.071 | - |
kaempferol | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
2.1.1.82 | 0.064 | - |
quercetin | mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
2.1.1.82 | 0.064 | - |
kaempferol | wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
2.1.1.82 | 0.07 | - |
kaempferol | mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
2.1.1.82 | 0.071 | - |
quercetin | wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.B75 | 7.5 | - |
assay at | Populus sp. |
2.1.1.B76 | 7.5 | - |
assay at | Populus sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.B75 | S-adenosyl-L-methionine | - |
Populus sp. | |
2.1.1.B76 | S-adenosyl-L-methionine | - |
Populus sp. |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.B75 | 1.7 | - |
quercetin | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
2.1.1.B75 | 1.9 | - |
kaempferol | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
2.1.1.B75 | 1.9 | - |
quercetin | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
2.1.1.B75 | 2.4 | - |
kaempferol | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
2.1.1.B76 | 1.7 | - |
3,4',5,7-tetrahydroxyflavanone | i.e. kaempferol, pH 7.5, 37°C, mutant enzyme N257G | Populus sp. | |
2.1.1.B76 | 1.7 | - |
quercetin | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
2.1.1.B76 | 1.9 | - |
3,4',5,7-tetrahydroxyflavanone | i.e. kaempferol, pH 7.5, 37°C, wild-type enzyme | Populus sp. | |
2.1.1.B76 | 1.9 | - |
2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-1-benzopyran-4-one | i.e. quercetin, pH 7.5, 37°C, mutant enzyme N257G | Populus sp. | |
2.1.1.B76 | 1.9 | - |
kaempferol | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
2.1.1.B76 | 1.9 | - |
quercetin | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
2.1.1.B76 | 2.4 | - |
2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-1-benzopyran-4-one | i.e. quercetin, pH 7.5, 37°C, wild-type enzyme | Populus sp. | |
2.1.1.B76 | 2.4 | - |
kaempferol | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
2.1.1.82 | 17 | - |
kaempferol | mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
2.1.1.82 | 19 | - |
quercetin | mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
2.1.1.82 | 19 | - |
kaempferol | wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
2.1.1.82 | 24 | - |
quercetin | wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. |