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Literature summary extracted from

  • Altenschmidt, U.; Oswald, B.; Fuchs, G.
    Purification and characterization of benzoate-coenzyme A ligase and 2-aminobenzoate-coenzyme A ligase from a denitrifying Pseudomonas sp. (1991), J. Bacteriol., 173, 5494-5501.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.25 5,5'-dithiobis(2-nitrobenzoate)
-
 Pseudomonas sp.
6.2.1.25 CuCl2
-
 Pseudomonas sp.
6.2.1.25 n-octylglucoside weak Pseudomonas sp.
6.2.1.25 p-chloromercuribenzoate
-
 Pseudomonas sp.
6.2.1.25 SDS
-
 Pseudomonas sp.
6.2.1.25 Tween 100
-
 Pseudomonas sp.
6.2.1.25 Zn2+
-
 Pseudomonas sp.
6.2.1.32 5,5'-dithiobis(2-nitrobenzoate) E2, no effect on isoenzyme E3 Pseudomonas sp.
6.2.1.32 Cu2+
-
 Pseudomonas sp.
6.2.1.32 n-octylglucoside
-
 Pseudomonas sp.
6.2.1.32 PCMB isoenzyme E3, no effect on E2 Pseudomonas sp.
6.2.1.32 SDS
-
 Pseudomonas sp.
6.2.1.32 Tween 100
-
 Pseudomonas sp.
6.2.1.32 Zn2+
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.25 0.011
-
 benzoate
-
 Pseudomonas sp.
6.2.1.25 0.1
-
 CoASH CoA Pseudomonas sp.
6.2.1.25 0.1
-
 CoASH 2-fluorobenzoate Pseudomonas sp.
6.2.1.25 0.13
-
 ATP
-
 Pseudomonas sp.
6.2.1.25 0.25
-
 2-Aminobenzoate
-
 Pseudomonas sp.
6.2.1.32 0.013
-
 2-Aminobenzoate isoenzyme E2 Pseudomonas sp.
6.2.1.32 0.015
-
 2-Aminobenzoate isoenzyme E3 Pseudomonas sp.
6.2.1.32 0.02
-
 CoA isoenzyme E2 Pseudomonas sp.
6.2.1.32 0.026
-
 CoA isoenzyme E3 Pseudomonas sp.
6.2.1.32 0.035
-
 benzoate isoenzyme E2 Pseudomonas sp.
6.2.1.32 0.04
-
 ATP isoenzyme E3 Pseudomonas sp.
6.2.1.32 0.05
-
 2-Fluorobenzoate isoenzyme E2 Pseudomonas sp.
6.2.1.32 0.07
-
 2-Fluorobenzoate isoenzyme E3 Pseudomonas sp.
6.2.1.32 0.075
-
 benzoate isoenzyme E3 Pseudomonas sp.
6.2.1.32 0.083
-
 ATP isoenzyme E2 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.25 Mg2+ required Pseudomonas sp.
6.2.1.25 Mn2+ can replace Mg2+ Pseudomonas sp.
6.2.1.32 Mg2+ required Pseudomonas sp.
6.2.1.32 Mn2+ required, can replace Mg2+ Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.32 60000
-
 isoenzyme E2, gel filtration Pseudomonas sp.
6.2.1.32 65000
-
 isoenzyme E3, gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.32 ATP + anthranilate + CoA Pseudomonas sp. isoenzyme E2 is induced anaerobically by 2-aminobenzoate and plays a role in anaerobic metabolism, E3 is induced aerobically by 2-aminobenzoate and is a key enzyme of aerobic metabolism AMP + diphosphate + anthranilyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.25 Pseudomonas sp.
-
-
-
6.2.1.25 Pseudomonas sp. KB740
-
-
-
6.2.1.32 Pseudomonas sp.
-
-
-
6.2.1.32 Pseudomonas sp. KB740
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
6.2.1.32 purified enzymes not sensitive to oxygen Pseudomonas sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.25
-
 Pseudomonas sp.
6.2.1.32 strain KB740, 2 forms, E2: MW 60000, induced anaerobically by 2-aminobenzoate and E3: MW 65000, induced aerobically by 2-aminobenzoate Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.32 culture condition:2-aminobenzoate-grown cell anaerob and nitrate Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.25 additional information
-
-
 Pseudomonas sp.
6.2.1.32 additional information
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
6.2.1.32 4°C, 0.05 mg/ml protein concentration, Tris-HCl buffer, pH 7.8, 50% loss of activity after 4 days Pseudomonas sp.
6.2.1.32 frozen in liquid nitrogen for 3 months without remarkable loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.25 ATP + 2-aminobenzoate + CoA 28% of the activity relative to benzoate Pseudomonas sp. AMP + diphosphate + 2-aminobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 2-aminobenzoate + CoA 28% of the activity relative to benzoate Pseudomonas sp. KB740 AMP + diphosphate + 2-aminobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 2-fluorobenzoate + CoA 120% of the activity relative to benzoate Pseudomonas sp. AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 2-fluorobenzoate + CoA 120% of the activity relative to benzoate Pseudomonas sp. KB740 AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 3-fluorobenzoate + CoA 76% of the activity relative to benzoate Pseudomonas sp. AMP + diphosphate + 3-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 3-fluorobenzoate + CoA 76% of the activity relative to benzoate Pseudomonas sp. KB740 AMP + diphosphate + 3-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 4-fluorobenzoate + CoA as effective as benzoate Pseudomonas sp. AMP + diphosphate + 4-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 4-fluorobenzoate + CoA as effective as benzoate Pseudomonas sp. KB740 AMP + diphosphate + 4-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + benzoate + CoA
-
 Pseudomonas sp. AMP + diphosphate + benzoyl-CoA
-
 ?
6.2.1.25 ATP + benzoate + CoA
-
 Pseudomonas sp. KB740 AMP + diphosphate + benzoyl-CoA
-
 ?
6.2.1.32 ATP + 2-fluorobenzoate + CoA at the same reaction rate as 2-aminobenzoate Pseudomonas sp. AMP + 2-fluorobenzoyl-CoA + diphosphate
-
 ?
6.2.1.32 ATP + 2-methylbenzoate + CoA at less than 1/5 of the rate as 2-aminobenzoate Pseudomonas sp. AMP + 2-methylbenzoyl-CoA + diphosphate
-
 ?
6.2.1.32 ATP + 3-fluorobenzoate + CoA at half the reaction rate as 2-aminobenzoate Pseudomonas sp. AMP + 3-fluorobenzoyl-CoA + diphosphate
-
 ?
6.2.1.32 ATP + 4-fluorobenzoate + CoA at the same reaction rate as 2-aminobenzoate Pseudomonas sp. AMP + 4-fluorobenzoyl-CoA + diphosphate
-
 ?
6.2.1.32 ATP + anthranilate + CoA isoenzyme E2 is induced anaerobically by 2-aminobenzoate and plays a role in anaerobic metabolism, E3 is induced aerobically by 2-aminobenzoate and is a key enzyme of aerobic metabolism Pseudomonas sp. AMP + diphosphate + anthranilyl-CoA
-
 ?
6.2.1.32 ATP + benzoate + CoA at the same reaction rate as 2-aminobenzoate Pseudomonas sp. AMP + benzoyl-CoA + diphosphate
-
 ?
6.2.1.32 ATP + CoA + anthranilate i.e. 2-aminobenzoate Pseudomonas sp. AMP + diphosphate + anthranilyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.32 monomer 1 * 60000, isoenzyme E2, 1 * 65000, isoenzyme E3, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.32 28
-
 assay at Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.25 433
-
 benzoate
-
 Pseudomonas sp.
6.2.1.32 21.7
-
 2-Aminobenzoate 28°C, isoenzyme E3 Pseudomonas sp.
6.2.1.32 267
-
 2-Aminobenzoate 28°C, isoenzyme E2 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.25 9.3
-
-
 Pseudomonas sp.
6.2.1.32 additional information
-
-
 Pseudomonas sp.
6.2.1.32 8.6
-
 isoenzyme E3 Pseudomonas sp.