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Literature summary extracted from

  • George, K.W.; Kagle, J.; Junker, L.; Risen, A.; Hay, A.G.
    Growth of Pseudomonas putida F1 on styrene requires increased catechol-2,3-dioxygenase activity, not a new hydrolase (2011), Microbiology, 157, 89-98.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.2 3-vinylcatechol C23O activity (TodE) is almost completely abrogated by exposure to 0.2 mM 3-vinylcatechol Pseudomonas putida
1.13.11.2 additional information C23O activity (TodE) is unaffected by styrene, toluene, and 3-methylcatechol Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2 ultracentrifugation and Prepease nickel resin chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-vinylcatechol + O2
-
 Pseudomonas putida ?
-
 ?
1.13.11.2 catechol + O2
-
 Pseudomonas putida 2-hydroxymuconate semialdehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.2 C23o
-
 Pseudomonas putida
1.13.11.2 TodE
-
 Pseudomonas putida

Expression

EC Number Organism Comment Expression
1.13.11.2 Pseudomonas putida C23O activity (TodE) is almost completely abrogated by exposure to 0.2 mM 3-vinylcatechol down
1.13.11.2 Pseudomonas putida C23O activity (TodE) is is unaffected by styrene, toluene, and 3-methylcatechol additional information

General Information

EC Number General Information Comment Organism
1.13.11.2 malfunction inactivation of TodE and the subsequent accumulation of 3-vinylcatechol results in toxicity and cell death Pseudomonas putida
1.13.11.2 physiological function overexpressing TodE is necessary to allow Pseudomonas putida strain F1 to grow on styrene Pseudomonas putida