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Literature summary extracted from

  • Case, C.L.; Rodriguez, J.R.; Mukhopadhyay, B.
    Characterization of an NADH oxidase of the flavin-dependent disulfide reductase family from Methanocaldococcus jannaschii (2009), Microbiology, 155, 69-79.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.3.3 expression in Escherichi coli C41 Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.3.3 1.9
-
 O2 pH 7.0, 83°C Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.3.3 50000
-
 x * 50000, SDS-PAGE Methanocaldococcus jannaschii
1.6.3.3 86000
-
 gel filtration Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
1.6.3.3 Methanocaldococcus jannaschii Q58065
-
-
1.8.1.14 no activity in Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.3.3
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.3.3 2 NADH + H+ + O2 the enzyme produces both H2O and H2O2. 62% of NADH-derived reducing equivalents are recovered as H2O2 and the rest probably generates H2O. The NADPH oxidase activity is about 5% compared to the activity with NADH Methanocaldococcus jannaschii 2 NAD+ + 2 H2O
-
 ?
1.6.3.3 NADPH + H+ + O2 the enzyme produces both H2O and H2O2. 62% of NADH-derived reducing equivalents are recovered as H2O2 and the rest probably generates H2O. The NADPH oxidase activity is about 5% compared to the activity with NADH Methanocaldococcus jannaschii NAD+ + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.3.3 ? x * 50000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
1.6.3.3 MJ0649
-
 Methanocaldococcus jannaschii
1.6.3.3 MJNox
-
 Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.3.3 FAD flavoprotein Methanocaldococcus jannaschii
1.6.3.3 NADH the NADPH oxidase activity is about 5% compared to the activity with NADH Methanocaldococcus jannaschii